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The significance of this second splicing pathway has intrigued RNA biologists for two decades, but its biological relevance was recently underscored when defects in the process were firmly linked to human disease. Here, we use a novel zebrafish mutant with defective minor class splicing to investigate how this pathway shapes the transcriptome during vertebrate development. We link its pleiotropic phenotype to widespread changes in gene expression that disrupt essential cellular pathways, including mRNA processing.<\/jats:p>","DOI":"10.1073\/pnas.1305536111","type":"journal-article","created":{"date-parts":[[2014,2,11]],"date-time":"2014-02-11T01:09:20Z","timestamp":1392080960000},"page":"3062-3067","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":67,"title":["Minor class splicing shapes the zebrafish transcriptome during development"],"prefix":"10.1073","volume":"111","author":[{"given":"Sebastian","family":"Markmiller","sequence":"first","affiliation":[{"name":"Ludwig Institute for Cancer Research, Melbourne-Parkville Branch, Parkville, VIC 3050, Australia;"},{"name":"Department of Surgery, Royal Melbourne Hospital, University of Melbourne, Parkville, VIC 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Cloonan","sequence":"additional","affiliation":[{"name":"Queensland Centre for Medical Genomics, Institute for Molecular Bioscience, University of Queensland, St. Lucia, QLD 4072, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rea M.","family":"Lardelli","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Melbourne-Parkville Branch, Parkville, VIC 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Doggett","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia;"},{"name":"Department of Medical Biology, University of Melbourne, Parkville, VIC 3052, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria-Cristina","family":"Keightley","sequence":"additional","affiliation":[{"name":"Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeliz","family":"Boglev","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Melbourne-Parkville Branch, Parkville, VIC 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Trotter","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Melbourne-Parkville Branch, Parkville, VIC 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie Y.","family":"Ng","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Melbourne-Parkville Branch, Parkville, VIC 3050, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon J.","family":"Wilkins","sequence":"additional","affiliation":[{"name":"Queensland Centre for Medical Genomics, Institute for Molecular Bioscience, University of Queensland, St. Lucia, QLD 4072, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Verkade","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Melbourne-Parkville Branch, Parkville, VIC 3050, Australia;"},{"name":"Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke A.","family":"Ober","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly A.","family":"Field","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean M.","family":"Grimmond","sequence":"additional","affiliation":[{"name":"Queensland Centre for Medical Genomics, Institute for Molecular Bioscience, University of Queensland, St. Lucia, QLD 4072, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham J.","family":"Lieschke","sequence":"additional","affiliation":[{"name":"Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier Y. 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