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Through genetic characterization of HIV-1 DNA in infected cells from peripheral blood and gut-associated lymphoid tissue from patients after long-term suppressive cART, our study reveals that the primary barrier to a cure is a remarkably stable pool of infected memory CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells. Through in-depth phylogenetic analyses, we determined that the HIV-1 reservoir in these cells from eight patients is kept stable during long-term cART and, with little evidence of viral replication, this population could be maintained by homeostatic cell proliferation or other processes.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1308313110","type":"journal-article","created":{"date-parts":[[2013,11,26]],"date-time":"2013-11-26T00:14:48Z","timestamp":1385424888000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":276,"title":["The HIV-1 reservoir in eight patients on long-term suppressive antiretroviral therapy is stable with few genetic changes over time"],"prefix":"10.1073","volume":"110","author":[{"given":"Lina","family":"Josefsson","sequence":"first","affiliation":[{"name":"Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82 Solna, Sweden;"},{"name":"Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 171 82 Solna, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"von Stockenstrom","sequence":"additional","affiliation":[{"name":"Department of Diagnostics and Vaccinology, Swedish Institute for Infectious Disease Control, 171 82 Solna, Sweden;"},{"name":"Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 171 82 Solna, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuno R.","family":"Faria","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Rega Institute, Katholieke Universiteit, 3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Sinclair","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, CA 94110;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Bacchetti","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics, University of California, San Francisco, CA 94143;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maudi","family":"Killian","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, CA 94110;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorrie","family":"Epling","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, CA 94110;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, CA 94110;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terence","family":"Ho","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, CA 94110;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Lemey","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Rega Institute, Katholieke Universiteit, 3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Shao","sequence":"additional","affiliation":[{"name":"Advanced Biomedical Computing Center, Science Applications International Corporation\u2013Frederick, Inc., National Cancer Institute\u2013Frederick, Frederick, MD 21702;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter W.","family":"Hunt","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, CA 94110;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma","family":"Somsouk","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Francisco, CA 94110;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Will","family":"Wylie","sequence":"additional","affiliation":[{"name":"Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; 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