{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:06:40Z","timestamp":1792721200394,"version":"4.5.0"},"reference-count":68,"publisher":"National Academy of Sciences","issue":"49","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2013,12,3]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    The vesicular trafficking pathways required for generation of the phagolysosome-like vacuole occupied by\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    are poorly defined, and no pathogen effectors of vesicular trafficking are known. Here, we reveal an important role for clathrin-mediated vesicular trafficking in\n                    <jats:italic>Coxiella<\/jats:italic>\n                    vacuole formation and identify a type 4B secretion system effector protein [\n                    <jats:italic>Coxiella<\/jats:italic>\n                    vacuolar protein A (CvpA)] that engages this pathway.\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>burnetii<\/jats:italic>\n                    CvpA traffics through the endocytic recycling compartment, and endocytic sorting motifs within CvpA bind the clathrin adaptor complex AP2. Mutation of\n                    <jats:italic>cvpA<\/jats:italic>\n                    , or depletion of AP2 or clathrin, significantly restricts\n                    <jats:italic>Coxiella<\/jats:italic>\n                    replication. Thus, our results reveal a effector\u2013clathrin interaction that benefits pathogen replication.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1309195110","type":"journal-article","created":{"date-parts":[[2013,11,18]],"date-time":"2013-11-18T23:49:39Z","timestamp":1384818579000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":97,"title":["<i>Coxiella burnetii<\/i>\n                    effector protein subverts clathrin-mediated vesicular trafficking for pathogen vacuole biogenesis"],"prefix":"10.1073","volume":"110","author":[{"given":"Charles L.","family":"Larson","sequence":"first","affiliation":[{"name":"Coxiella Pathogenesis Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul 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