{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:55:02Z","timestamp":1790852102047,"version":"4.1.0"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"29","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2013,7,16]]},"abstract":"\n Excitation\u2013contraction (EC) coupling comprises events in muscle that convert electrical signals to Ca\n 2+<\/jats:sup>\n transients, which then trigger contraction of the sarcomere. Defects in these processes cause a spectrum of muscle diseases. We report that STAC3, a skeletal muscle-specific protein that localizes to T tubules, is essential for coupling membrane depolarization to Ca\n 2+<\/jats:sup>\n release from the sarcoplasmic reticulum (SR). Consequently, homozygous deletion of src homology 3 and cysteine rich domain 3 (\n Stac3<\/jats:italic>\n ) in mice results in complete paralysis and perinatal lethality with a range of musculoskeletal defects that reflect a blockade of EC coupling. Muscle contractility and Ca\n 2+<\/jats:sup>\n release from the SR of cultured myotubes from\n Stac3<\/jats:italic>\n mutant mice could be restored by application of 4-chloro-\n m<\/jats:italic>\n -cresol, a ryanodine receptor agonist, indicating that the sarcomeres, SR Ca\n 2+<\/jats:sup>\n store, and ryanodine receptors are functional in\n Stac3<\/jats:italic>\n mutant skeletal muscle. These findings reveal a previously uncharacterized, but required, component of the EC coupling machinery of skeletal muscle and introduce a candidate for consideration in myopathic disorders.\n <\/jats:p>","DOI":"10.1073\/pnas.1310571110","type":"journal-article","created":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T00:42:22Z","timestamp":1372725742000},"page":"11881-11886","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":142,"title":["Skeletal muscle-specific T-tubule protein STAC3 mediates voltage-induced Ca\n 2+<\/sup>\n release and contractility"],"prefix":"10.1073","volume":"110","author":[{"given":"Benjamin R.","family":"Nelson","sequence":"first","affiliation":[{"name":"Departments of aMolecular Biology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenfen","family":"Wu","sequence":"additional","affiliation":[{"name":"Neurology and Neurotherapeutics, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Liu","sequence":"additional","affiliation":[{"name":"Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas M.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Departments of aMolecular Biology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"McAnally","sequence":"additional","affiliation":[{"name":"Departments of aMolecular Biology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weichun","family":"Lin","sequence":"additional","affiliation":[{"name":"Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen C.","family":"Cannon","sequence":"additional","affiliation":[{"name":"Neurology and Neurotherapeutics, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhonda","family":"Bassel-Duby","sequence":"additional","affiliation":[{"name":"Departments of aMolecular Biology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric N.","family":"Olson","sequence":"additional","affiliation":[{"name":"Departments of aMolecular Biology,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2013,7]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/346567a0"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2587"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/415198a"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/380072a0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404836101"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307538200"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.101618098"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi972907o"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.14.7791"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200910241"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205866200"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M709231200"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0005660"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3791\/1520"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.202804"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/364501a0"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-02-00539.2003"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81251-9"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004956107"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(65)90038-2"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/369556a0"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.24.13961"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709312104"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.10.010"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2792"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(84)90127-1"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(84)90128-3"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.361311"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062760"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.018119"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2000.88.2.573"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI57398"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1310571110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T21:42:00Z","timestamp":1649799720000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1310571110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7]]},"references-count":33,"journal-issue":{"issue":"29","published-print":{"date-parts":[[2013,7,16]]}},"alternative-id":["10.1073\/pnas.1310571110"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1310571110","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.718030155.793481637","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7]]},"assertion":[{"value":"2013-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}