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This study reveals a series of structural snapshots of GALC captured during different steps of the catalytic cycle. These structures identify specific residues within the active site that undergo significant movements during substrate cleavage, providing key insight into the catalytic mechanism of GALC.<\/jats:p>","DOI":"10.1073\/pnas.1311990110","type":"journal-article","created":{"date-parts":[[2013,12,2]],"date-time":"2013-12-02T23:57:30Z","timestamp":1386028650000},"page":"20479-20484","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":49,"title":["Structural snapshots illustrate the catalytic cycle of \u03b2-galactocerebrosidase, the defective enzyme in Krabbe disease"],"prefix":"10.1073","volume":"110","author":[{"given":"Chris H.","family":"Hill","sequence":"first","affiliation":[{"name":"Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen C.","family":"Graham","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randy J.","family":"Read","sequence":"additional","affiliation":[{"name":"Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet E.","family":"Deane","sequence":"additional","affiliation":[{"name":"Department of Haematology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2013,12,2]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0009-8981(98)00095-3","article-title":"Expression and processing of recombinant human galactosylceramidase","volume":"276","author":"Nagano S","year":"1998","unstructured":"S Nagano, et al., Expression and processing of recombinant human galactosylceramidase. 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