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We observed by Western blot analyses that ablation of AgRP neurons results in enhanced expression of NR2B. Interestingly, systemic administration of LiCl in a critical time window before AgRP neuron ablation abolished the anorectic response. LiCl treatment suppressed NR2B levels in the PBN and ameliorated the local Fos induction that is associated with anorexia. This protective role of LiCl on feeding was blunted in vagotomized mice. Chronic infusion of RO25-6981, a selective NR2B inhibitor, into the PBN recapitulated the role of LiCl in maintaining feeding after AgRP neuron ablation. We suggest that the accumulation of NR2B subunits in the PBN contributes to aphagia in response to AgRP neuron ablation and may be involved in other forms of anorexia.<\/jats:p>","DOI":"10.1073\/pnas.1314137110","type":"journal-article","created":{"date-parts":[[2013,8,20]],"date-time":"2013-08-20T22:34:43Z","timestamp":1377038083000},"page":"14765-14770","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":33,"title":["NR2B subunit of the NMDA glutamate receptor regulates appetite in the parabrachial nucleus"],"prefix":"10.1073","volume":"110","author":[{"given":"Qi","family":"Wu","sequence":"first","affiliation":[{"name":"Departments of aBiochemistry and"},{"name":"Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA 98195; and"},{"name":"Department of Pharmacology and"},{"name":"Eagles Diabetes Research Center, Carver College of Medicine, University of Iowa, Iowa City, IA 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruimao","family":"Zheng","sequence":"additional","affiliation":[{"name":"Pharmacology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dollada","family":"Srisai","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and"},{"name":"Eagles Diabetes Research Center, Carver College of Medicine, University of Iowa, Iowa City, IA 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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