{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T06:59:12Z","timestamp":1793343552951,"version":"4.5.0"},"reference-count":49,"publisher":"National Academy of Sciences","issue":"48","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2013,11,26]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>There is an enormous interest in the mechanism by which proteins misfold and aggregate into amyloid fibrils. Amyloid has been implicated in many human diseases, but the mechanism of aggregation is not understood. Intermediates have been postulated to play an important role in the process, but there have been very few direct measurements that provide specific structural details. The use of isotope labeling and 2D IR methods has allowed the characterization of a critical intermediate generated during amyloid formation by islet amyloid polypeptide, the peptide responsible for amyloid formation in type 2 diabetes. Identification of this intermediate provides a structural explanation for the lag phase and may explain why some species develop amyloid deposits of hIAPP while others do not.<\/jats:p>","DOI":"10.1073\/pnas.1314481110","type":"journal-article","created":{"date-parts":[[2013,11,11]],"date-time":"2013-11-11T23:48:45Z","timestamp":1384213725000},"page":"19285-19290","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":277,"title":["Mechanism of IAPP amyloid fibril formation involves an intermediate with a transient \u03b2-sheet"],"prefix":"10.1073","volume":"110","author":[{"given":"Lauren E.","family":"Buchanan","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Wisconsin\u2013Madison, Madison, WI 53706-1396;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily B.","family":"Dunkelberger","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Wisconsin\u2013Madison, Madison, WI 53706-1396;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huong Q.","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Wisconsin\u2013Madison, Madison, WI 53706-1396;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin-Nan","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of California, Irvine, CA 92697-2025;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Cheng","family":"Chiu","sequence":"additional","affiliation":[{"name":"Institute for Molecular Engineering, The University of Chicago, Chicago, IL 60637;"},{"name":"Argonne National Laboratory, Lemont, IL 60439; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Chemistry, State University of New York, Stony Brook, NY 11794-3400"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Raleigh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, State University of New York, Stony Brook, NY 11794-3400"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan J.","family":"de Pablo","sequence":"additional","affiliation":[{"name":"Institute for Molecular Engineering, The University of Chicago, Chicago, IL 60637;"},{"name":"Argonne National Laboratory, Lemont, IL 60439; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James S.","family":"Nowick","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of California, Irvine, CA 92697-2025;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin T.","family":"Zanni","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Wisconsin\u2013Madison, Madison, WI 53706-1396;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2013,11,11]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.101304.123901"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.9.2304"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1079469"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi701427q"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.036509.108"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C300049200"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja1089827"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1213151"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi400027y"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1203193109"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4970"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2013.01.046"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)81467-X"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.13.5036"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3422"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00028a073"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(88)80538-2"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.104.047076"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1063\/1.3097982"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2009.03.039"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4593"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3286"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi048582a"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja072157y"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507471103"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1293"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802993105"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909888106"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805957106"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja204035k"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1237107100"},{"key":"e_1_3_3_32_2","first-page":"81","article-title":"Formation of amyloid fibrils in vitro by human gammaD-crystallin and its isolated domains","volume":"14","author":"Papanikolopoulou K","year":"2008","unstructured":"K Papanikolopoulou, et al., Formation of amyloid fibrils in vitro by human gammaD-crystallin and its isolated domains. Mol Vis 14, 81\u201389 (2008).","journal-title":"Mol Vis"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja110545h"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.1433"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.202427399"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp067873l"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi101936c"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.04.016"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0915166107"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05695"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2010.01.006"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja1046186"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar200189b"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.07.077"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711664105"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1178250"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218402110"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol101981b"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2010.05.002"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1314481110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T22:48:09Z","timestamp":1649803689000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1314481110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,11]]},"references-count":49,"journal-issue":{"issue":"48","published-print":{"date-parts":[[2013,11,26]]}},"alternative-id":["10.1073\/pnas.1314481110"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1314481110","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,11]]},"assertion":[{"value":"2013-11-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}