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In this study, we developed a safety mechanism for VACV based on the conditional expression of a protective gene (IFN-\u03b3) under a tightly controlled tetracycline-inducible promoter. We showed that immunodeficient mice are protected from a lethal VACV infection when treated with tetracyclines. This strategy has the potential to allow treatment of any complications that may result from VACV replication to be as simple as standard antibiotic regimens. Such \u201cSMART\u201d VACV vectors have broad applications in the fields of vaccinology and cancer therapy.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1314483110","type":"journal-article","created":{"date-parts":[[2013,8,30]],"date-time":"2013-08-30T00:29:18Z","timestamp":1377822558000},"page":"15407-15412","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":11,"title":["Safety mechanism assisted by the repressor of tetracycline (SMART) vaccinia virus vectors for vaccines and therapeutics"],"prefix":"10.1073","volume":"110","author":[{"given":"Patricia","family":"Grigg","sequence":"first","affiliation":[{"name":"International Laboratory of Molecular Biology for Tropical Disease Agents, School of Veterinary Medicine, University of California, Davis, CA 95616;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison","family":"Titong","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Veterinary Science and Center of Excellence for Vaccine Research, College of Agriculture and Natural Resources, University of Connecticut, Storrs, CT 06269; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie A.","family":"Jones","sequence":"additional","affiliation":[{"name":"International Laboratory of Molecular Biology for Tropical Disease Agents, School of Veterinary Medicine, University of California, Davis, CA 95616;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tilahun D.","family":"Yilma","sequence":"additional","affiliation":[{"name":"International Laboratory of Molecular Biology for Tropical Disease Agents, School of Veterinary Medicine, University of California, Davis, CA 95616;"},{"name":"Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo H.","family":"Verardi","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Veterinary Science and Center of Excellence for Vaccine Research, College of Agriculture and Natural Resources, University of Connecticut, Storrs, CT 06269; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2013,8,29]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"1","article-title":"Viral vectors: A look back and ahead on gene transfer technology","volume":"36","author":"Vannucci L","year":"2013","unstructured":"L Vannucci, M Lai, F Chiuppesi, L Ceccherini-Nelli, M Pistello, Viral vectors: A look back and ahead on gene transfer technology. 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