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U.S.A."],"published-print":{"date-parts":[[2014,1,28]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    When hornworm (\n                    <jats:italic>Manduca sexta<\/jats:italic>\n                    ) larvae feed on\n                    <jats:italic>Nicotiana attenuata<\/jats:italic>\n                    plants in native habitats, more disappear at night when they feed on transgenic, nicotine-free\n                    <jats:italic>N. attenuata<\/jats:italic>\n                    plants because wolf spiders (\n                    <jats:italic>Camptocosa parallela<\/jats:italic>\n                    ) selectively prey on nicotine-free larvae. When larvae consume nicotine-replete plants, their midgut-expressed cytochrome P450 6B46 (\n                    <jats:italic>CYP6B46<\/jats:italic>\n                    ) is upregulated. The larvae in which\n                    <jats:italic>CYP6B46<\/jats:italic>\n                    is silenced by plant-mediated RNAi excrete more of their ingested nicotine. Silencing\n                    <jats:italic>CYP6B46<\/jats:italic>\n                    impairs the mechanisms of passing ingested nicotine from the midgut to the hemolymph to be exhaled from the spiracles during spider attack. Spiders are deterred by this nicotine-rich halitosis. Thus, the\n                    <jats:italic>CYP6B46<\/jats:italic>\n                    -silenced larvae exhale less nicotine and become ready spider prey, demonstrating that\n                    <jats:italic>CYP6B46<\/jats:italic>\n                    functions to repurpose the normally excreted nicotine for defense.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1314848111","type":"journal-article","created":{"date-parts":[[2013,12,30]],"date-time":"2013-12-30T21:43:06Z","timestamp":1388439786000},"page":"1245-1252","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":127,"title":["Natural history-driven, plant-mediated RNAi-based study reveals\n                    <i>CYP6B46<\/i>\n                    \u2019s role in a nicotine-mediated antipredator herbivore defense"],"prefix":"10.1073","volume":"111","author":[{"given":"Pavan","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Molecular Ecology, 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