{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:15:57Z","timestamp":1792728957904,"version":"4.5.0"},"reference-count":89,"publisher":"National Academy of Sciences","issue":"39","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2013,9,24]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    The evolution of multidrug resistance in pathogenic bacteria, including uropathogenic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (UPEC), that cause most urinary tract infections is becoming a worldwide crisis. UPEC use a variety of virulence factors and adhesins, including the mannose-binding FimH adhesin, to colonize and invade bladder tissue, often forming intracellular biofilms and quiescent reservoirs that can contribute to recurrent infections recalcitrant to treatment. Using two prototypical UPEC strains, we discovered that positively selected residues outside of the FimH mannose-binding pocket affect transitions between low- and high-affinity FimH conformations, which extraordinarily impacts FimH function during pathogenesis. Thus, this work elucidates mechanistic and functional insights into pathoadaptation and evolutionary fine-tuning of critical virulence interactions.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1315203110","type":"journal-article","created":{"date-parts":[[2013,9,3]],"date-time":"2013-09-03T21:58:16Z","timestamp":1378245496000},"page":"15530-15537","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":143,"title":["Positively selected FimH residues enhance virulence during urinary tract infection by altering FimH conformation"],"prefix":"10.1073","volume":"110","author":[{"given":"Drew J.","family":"Schwartz","sequence":"first","affiliation":[{"name":"Center for Women\u2019s Infectious Disease Research, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasilios","family":"Kalas","sequence":"additional","affiliation":[{"name":"Center for Women\u2019s Infectious Disease Research, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110; 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