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Here we demonstrate that O-mannosylation, an essential posttranslational protein modification, is crucial for the formation of adherens junctions between cells of the early mouse embryo, with O-mannosylation\u2013deficient embryos dying at the morula-to-blastocyst transition. Moreover, we identified O-mannosyl glycans on E-cadherin, the major cell\u2013cell adhesion protein of embryos, and demonstrated that these glycans are crucial for E-cadherin\u2013mediated cell adhesion. Because O-mannosylation is a conserved feature of the classical cadherins, this protein modification most likely affects many more biological processes than previously thought.<\/jats:p>","DOI":"10.1073\/pnas.1316753110","type":"journal-article","created":{"date-parts":[[2013,12,2]],"date-time":"2013-12-02T23:57:30Z","timestamp":1386028650000},"page":"21024-21029","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":85,"title":["Protein O-mannosylation is crucial for E-cadherin\u2013mediated cell adhesion"],"prefix":"10.1073","volume":"110","author":[{"given":"Mark","family":"Lommel","sequence":"first","affiliation":[{"name":"Centre for Organismal Studies, Cell Chemistry and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick R.","family":"Winterhalter","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Cell Chemistry and"},{"name":"Center for Molecular Biology, University of Heidelberg, 69120 Heidelberg, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Willer","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Cell Chemistry and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maik","family":"Dahlhoff","sequence":"additional","affiliation":[{"name":"Gene Center, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlon R.","family":"Schneider","sequence":"additional","affiliation":[{"name":"Gene Center, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus F.","family":"Bartels","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Cell Chemistry and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Renner-M\u00fcller","sequence":"additional","affiliation":[{"name":"Gene Center, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Ruppert","sequence":"additional","affiliation":[{"name":"Center for Molecular Biology, University of Heidelberg, 69120 Heidelberg, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eckhard","family":"Wolf","sequence":"additional","affiliation":[{"name":"Gene Center, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Strahl","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies, Cell Chemistry and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2013,12,2]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwp066"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2011.02.001"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02814"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405899101"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00837"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00572.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.203281"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2009.044"},{"key":"e_1_3_3_9_2","unstructured":"JC Trinidad R Schoepfer AL Burlingame KF Medzihradszky N- and O-glycosylation in the murine synaptosome. 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