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By determining the crystal structure of Sas-4\u2018s C-terminal T complex protein 10 (TCP) domain and functional studies in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    , human cells, and induced pluripotent stem cell-derived neural progenitors, we show that Sas-4 performs its tethering role via its TCP domain. Furthermore, point mutations within the TCP domain perturb PCM tethering while still allowing the protein to scaffold cytoplasmic PCM complexes. These studies provide insights into how Sas-4 proteins tether PCM complexes for the assembly of functional centrosomes.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1317535111","type":"journal-article","created":{"date-parts":[[2014,1,2]],"date-time":"2014-01-02T22:31:32Z","timestamp":1388701892000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":69,"title":["Conserved TCP domain of Sas-4\/CPAP is essential for pericentriolar material tethering during centrosome biogenesis"],"prefix":"10.1073","volume":"111","author":[{"given":"Xiangdong","family":"Zheng","sequence":"first","affiliation":[{"name":"Ministry of Education Key Laboratory of Protein Sciences, Center for Structural Biology, School of Life Sciences, and"},{"name":"Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, People\u2019s Republic of China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li Ming","family":"Gooi","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I and Center for Molecular Medicine, University of Cologne, 50931 Cologne, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arpit","family":"Wason","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I and Center for Molecular Medicine, University of Cologne, 50931 Cologne, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elke","family":"Gabriel","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I and Center for Molecular Medicine, University of Cologne, 50931 Cologne, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narges Zare","family":"Mehrjardi","sequence":"additional","affiliation":[{"name":"Institute for Neurophysiology, Medical Center, University of Cologne, 50931 Cologne, Germany;"},{"name":"Department of Developmental Biology, University of Science and Culture, Academic Center for Education Culture and Research, 19395-4644 Tehran, Iran;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Yang","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory of Protein Sciences, Center for Structural Biology, School of Life Sciences, and"},{"name":"Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, People\u2019s Republic of China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingrun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory of Protein Sciences, Center for Structural Biology, School of Life Sciences, and"},{"name":"Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, People\u2019s Republic of China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Debec","sequence":"additional","affiliation":[{"name":"Polarity and Morphogenesis Group, Institut Jacques Monod, Centre National de la Recherche Scientifique, University Paris Diderot, 75013 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus L.","family":"Basiri","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Toledo, Toledo, OH 43606; 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