{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T12:07:53Z","timestamp":1791029273990,"version":"4.1.0"},"reference-count":48,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,6,25]]},"abstract":"<jats:p>\n                    Ischemia\/reperfusion (I\/R) injury is a serious potential threat to outcomes in organ transplantation and other clinical arenas in which there is temporary interruption of blood flow. I\/R is a frequent cause of primary failure in organ transplantation. We hypothesized that the antioxidant bucillamine, a potent sulfhydryl donor, would protect against I\/R injury in high-risk organ transplants. Because livers subjected to prolonged ischemia and very fatty livers are highly susceptible to severe I\/R injury, we studied the effect of bucillamine in three animal models of liver transplantation: two\n                    <jats:italic>ex vivo<\/jats:italic>\n                    models of isolated perfused livers, either normal or fatty rat livers, and an\n                    <jats:italic>in vivo<\/jats:italic>\n                    model of syngenic orthotopic liver transplants in rats. In all models, livers were deprived of oxygen for 24 h before either\n                    <jats:italic>ex vivo<\/jats:italic>\n                    reperfusion or transplantation. In the\n                    <jats:italic>ex vivo<\/jats:italic>\n                    models, bucillamine treatment significantly improved portal vein blood flow and bile production, preserved normal liver architecture, and significantly reduced liver enzyme release and indices of oxidative stress. Moreover, bucillamine treatment significantly increased levels of reduced glutathione in the liver and lowered levels of oxidized glutathione in both liver and blood. In rats subjected to liver transplants, bucillamine significantly enhanced survival and protected against hepatic injury. Possible mechanisms of this protection include prevention of excessive accumulation of toxic oxygen species, interruption of redox signaling in hepatocytes, and inhibition of macrophage activation. This study demonstrates the potential utility of bucillamine or other cysteine-derived thiol donors for improving outcomes in organ transplantation and other clinical settings involving I\/R injury.\n                  <\/jats:p>","DOI":"10.1073\/pnas.132026099","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T18:26:28Z","timestamp":1027880788000},"page":"8915-8920","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":55,"title":["Bucillamine, a thiol antioxidant, prevents transplantation-associated reperfusion injury"],"prefix":"10.1073","volume":"99","author":[{"given":"Farin","family":"Amersi","sequence":"first","affiliation":[{"name":"The Dumont-University of California, Los Angeles Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, Los Angeles, CA 90095; Webb\u2013Waring Antioxidant Research Institute, Denver, CO, 80262; Division of Cardiology, Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262; and Division of Infectious Diseases, Department of Medicine, 37-121 CHS, University of California, Los Angeles School of Medicine, 10833 Le Conte Avenue, Los Angeles,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally K.","family":"Nelson","sequence":"additional","affiliation":[{"name":"The Dumont-University of California, Los Angeles Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, Los Angeles, CA 90095; Webb\u2013Waring Antioxidant Research Institute, Denver, CO, 80262; Division of Cardiology, Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262; and Division of Infectious Diseases, Department of Medicine, 37-121 CHS, University of California, Los Angeles School of Medicine, 10833 Le Conte Avenue, Los Angeles,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu Da","family":"Shen","sequence":"additional","affiliation":[{"name":"The Dumont-University of California, Los Angeles Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, Los Angeles, CA 90095; Webb\u2013Waring Antioxidant Research Institute, Denver, CO, 80262; Division of Cardiology, Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262; and Division of Infectious Diseases, Department of Medicine, 37-121 CHS, University of California, Los Angeles School of Medicine, 10833 Le Conte Avenue, Los Angeles,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirohisa","family":"Kato","sequence":"additional","affiliation":[{"name":"The Dumont-University of California, Los Angeles Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, Los Angeles, CA 90095; Webb\u2013Waring Antioxidant Research Institute, Denver, CO, 80262; Division of Cardiology, Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262; and Division of Infectious Diseases, Department of Medicine, 37-121 CHS, University of California, Los Angeles School of Medicine, 10833 Le Conte Avenue, Los Angeles,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy","family":"Melinek","sequence":"additional","affiliation":[{"name":"The Dumont-University of California, Los Angeles Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, Los Angeles, CA 90095; Webb\u2013Waring Antioxidant Research Institute, Denver, CO, 80262; Division of Cardiology, Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262; and Division of Infectious Diseases, Department of Medicine, 37-121 CHS, University of California, Los Angeles School of Medicine, 10833 Le Conte Avenue, Los Angeles,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerzy W.","family":"Kupiec-Weglinski","sequence":"additional","affiliation":[{"name":"The Dumont-University of California, Los Angeles Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, Los Angeles, CA 90095; Webb\u2013Waring Antioxidant Research Institute, Denver, CO, 80262; Division of Cardiology, Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262; and Division of Infectious Diseases, Department of Medicine, 37-121 CHS, University of California, Los Angeles School of Medicine, 10833 Le Conte Avenue, Los Angeles,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence D.","family":"Horwitz","sequence":"additional","affiliation":[{"name":"The Dumont-University of California, Los Angeles Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, Los Angeles, CA 90095; Webb\u2013Waring Antioxidant Research Institute, Denver, CO, 80262; Division of Cardiology, Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262; and Division of Infectious Diseases, Department of Medicine, 37-121 CHS, University of California, Los Angeles School of Medicine, 10833 Le Conte Avenue, Los Angeles,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald W.","family":"Busuttil","sequence":"additional","affiliation":[{"name":"The Dumont-University of California, Los Angeles Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, Los Angeles, CA 90095; Webb\u2013Waring Antioxidant Research Institute, Denver, CO, 80262; Division of Cardiology, Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262; and Division of Infectious Diseases, Department of Medicine, 37-121 CHS, University of California, Los Angeles School of Medicine, 10833 Le Conte Avenue, Los Angeles,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus A.","family":"Horwitz","sequence":"additional","affiliation":[{"name":"The Dumont-University of California, Los Angeles Transplant Center, Division of Liver and Pancreas Transplantation, Department of Surgery, Los Angeles, CA 90095; 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