{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:11:19Z","timestamp":1792541479685,"version":"4.5.0"},"reference-count":24,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Carbapenem-resistant\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    has emerged globally as a multidrug-resistant hospital pathogen for which there are few treatment options. Clinical isolates classified by multilocus sequence typing (ST) as ST258 are the most widespread. The basis for the success of ST258 organisms above and beyond antibiotic resistance is not known, nor is it clear whether infections are caused by a single clone. We used genome sequencing to reveal unexpected genetic diversity among ST258 organisms (thus disproving the single-clone hypothesis) and identified a recombination hotspot that accounts for the majority of divergence\u2014and presumably for serologic variation\u2014among ST258 clinical isolates. Our findings will facilitate the development of new clinical strategies designed to prevent or treat infections caused by multidrug-resistant\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.1321364111","type":"journal-article","created":{"date-parts":[[2014,3,18]],"date-time":"2014-03-18T01:41:09Z","timestamp":1395106869000},"page":"4988-4993","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":330,"title":["Molecular dissection of the evolution of carbapenem-resistant multilocus sequence type 258\n                    <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1073","volume":"111","author":[{"given":"Frank R.","family":"DeLeo","sequence":"first","affiliation":[{"name":"Laboratory of Human Bacterial Pathogenesis and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"name":"Public Health Research Institute Tuberculosis Center, New Jersey Medical School-Rutgers University, Newark, NJ 07103;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen F.","family":"Porcella","sequence":"additional","affiliation":[{"name":"Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig A.","family":"Martens","sequence":"additional","affiliation":[{"name":"Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott D.","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Human Bacterial Pathogenesis and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeline R.","family":"Porter","sequence":"additional","affiliation":[{"name":"Laboratory of Human Bacterial Pathogenesis and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalyan D.","family":"Chavda","sequence":"additional","affiliation":[{"name":"Public Health Research Institute Tuberculosis Center, New Jersey Medical School-Rutgers University, Newark, NJ 07103;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Department of dPathology and of"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barun","family":"Mathema","sequence":"additional","affiliation":[{"name":"Public Health Research Institute Tuberculosis Center, New Jersey Medical School-Rutgers University, Newark, NJ 07103;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randall J.","family":"Olsen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Genomic Medicine and"},{"name":"Center for Human Molecular and Translational Infectious Diseases, Houston Methodist Research Institute, Houston Methodist Hospital System, Houston, TX 77204; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Pharmacology, Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, OH 44106;"},{"name":"Research Service, Louis Stokes Cleveland Veterans Affairs Medical Center, Cleveland, OH 44106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"Musser","sequence":"additional","affiliation":[{"name":"Department of Pathology and Genomic Medicine and"},{"name":"Center for Human Molecular and Translational Infectious Diseases, Houston Methodist Research Institute, Houston Methodist Hospital System, Houston, TX 77204; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry N.","family":"Kreiswirth","sequence":"additional","affiliation":[{"name":"Public Health Research Institute Tuberculosis Center, New Jersey Medical School-Rutgers University, Newark, NJ 07103;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,3,17]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/S1473-3099(13)70190-7","article-title":"Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases","volume":"13","author":"Munoz-Price LS","year":"2013","unstructured":"LS Munoz-Price, et al., Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases. 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