{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:50:06Z","timestamp":1790895006148,"version":"4.1.0"},"reference-count":30,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,6,24]]},"abstract":"<jats:p>A recombinant S segment RNA (Sr) of the prototypic arenavirus lymphocytic  choriomeningitis virus (LCMV) where the glycoprotein of vesicular stomatitis  virus (VSVG) was substituted for the glycoprotein of LCMV (LCMV-GP) was  produced intracellularly from cDNA under the control of a polymerase I  promoter. Coexpression of the LCMV proteins NP and L allowed expression of  VSVG from Sr. Infection of transfected cells with WT LCMV (LCMVwt) resulted in  reassortment of the L segment of LCMVwt with the Sr at low frequency.  Isolation of recombinant LCMV (rLCMV) expressing VSVG (rLCMV\/VSVG) was  achieved by selection against LCMVwt by using a cell line deficient in the  cellular protease S1P. This approach was based on the finding that processing  of LCMV-GP by S1P was required for virus infectivity. Characterization of  protein and RNA expression of rLCMV\/VSVG in infected cells confirmed the  expected virus genome organization. rLCMV\/VSVG caused syncytium formation in  cultured cells and grew to \u2248100-fold lower titers than WT virus but, like  the parent virus, it persisted in neonatally infected mice without clinical  signs of disease.<\/jats:p>","DOI":"10.1073\/pnas.1332709100","type":"journal-article","created":{"date-parts":[[2003,6,24]],"date-time":"2003-06-24T13:47:39Z","timestamp":1056462459000},"page":"7895-7900","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":74,"title":["Recombinant lymphocytic choriomeningitis virus expressing vesicular  stomatitis virus glycoprotein"],"prefix":"10.1073","volume":"100","author":[{"given":"Daniel D.","family":"Pinschewer","sequence":"first","affiliation":[{"name":"Department of Neuropharmacology, The Scripps Research Institute, IMM6,  10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar","family":"Perez","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology, The Scripps Research Institute, IMM6,  10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana B.","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology, The Scripps Research Institute, IMM6,  10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"de la Torre","sequence":"additional","affiliation":[{"name":"Department of Neuropharmacology, The Scripps Research Institute, IMM6,  10550 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2003,6,13]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/300360a0"},{"key":"e_1_3_2_2_2","first-page":"479","volume":"37","year":"1962","unstructured":"Hotchin, J. 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