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This study reveals a role for the JAK2V617F mutation, the most frequent genetic abnormality in MPN patients, in impairing replication fork progression during cell division of MPN patient-derived tumor cells. Moreover, analysis of different MPN disease subtypes reveals unexpected differences in DNA repair activity in response to JAK2V617F-induced perturbations in replication dynamics. These findings have potential implications for tumor clonal evolution and individualized cancer therapy.<\/jats:p>","DOI":"10.1073\/pnas.1401873111","type":"journal-article","created":{"date-parts":[[2014,10,6]],"date-time":"2014-10-06T22:54:30Z","timestamp":1412636070000},"page":"15190-15195","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":46,"title":["JAK2V617F promotes replication fork stalling with disease-restricted impairment of the intra-S checkpoint response"],"prefix":"10.1073","volume":"111","author":[{"given":"Edwin","family":"Chen","sequence":"first","affiliation":[{"name":"Cambridge Institute for Medical Research,"},{"name":"Medical Research Council\/Wellcome Trust Cambridge Stem Cell Institute, and"},{"name":"Department of Haematology, University of Cambridge, Cambridge CB2 0XY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Sook","family":"Ahn","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research,"},{"name":"Medical Research Council\/Wellcome Trust Cambridge Stem Cell Institute, and"},{"name":"Department of Haematology, University of Cambridge, Cambridge CB2 0XY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlie E.","family":"Massie","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research,"},{"name":"Medical Research Council\/Wellcome Trust Cambridge Stem Cell Institute, and"},{"name":"Department of Haematology, University of Cambridge, Cambridge CB2 0XY, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Clynes","sequence":"additional","affiliation":[{"name":"The Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna L.","family":"Godfrey","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research,"},{"name":"Medical Research Council\/Wellcome Trust Cambridge Stem Cell Institute, and"},{"name":"Department of Haematology, University of Cambridge, Cambridge CB2 0XY, United Kingdom;"},{"name":"Department of Haematology, Addenbrooke\u2019s Hospital, Cambridge CB2 0XY, United Kingdom; 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