{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:03:34Z","timestamp":1792670614862,"version":"4.5.0"},"reference-count":35,"publisher":"National Academy of Sciences","issue":"15","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,7,18]]},"abstract":"\n (\n RS<\/jats:italic>\n )-2-\n cis<\/jats:italic>\n , 4-\n trans<\/jats:italic>\n -abscisic acid (ABA), a naturally occurring plant stress hormone, elicited rapid agonist-specific changes in\n myo<\/jats:italic>\n -inositol hexakisphosphate (InsP\n 6<\/jats:sub>\n ) measured in intact guard cells of\n Solanum tuberosum<\/jats:italic>\n (\n n<\/jats:italic>\n = 5); these changes were not reproduced by (\n RS<\/jats:italic>\n )-2-\n trans<\/jats:italic>\n , 4-\n trans<\/jats:italic>\n -abscisic acid, an inactive stereoisomer of ABA (\n n<\/jats:italic>\n = 4). The electrophysiological effects of InsP\n 6<\/jats:sub>\n were assessed on both\n S. tuberosum<\/jats:italic>\n (\n n<\/jats:italic>\n = 14) and\n Vicia faba<\/jats:italic>\n (\n n<\/jats:italic>\n = 6) guard cell protoplasts. In both species, submicromolar concentrations of InsP\n 6<\/jats:sub>\n , delivered through the patch electrode, mimicked the inhibitory effects of ABA and internal calcium (Ca\n i<\/jats:sub>\n 2+<\/jats:sup>\n ) on the inward rectifying K\n +<\/jats:sup>\n current,\n I<\/jats:italic>\n K,in<\/jats:sub>\n , in a dose-dependent manner. Steady state block of\n I<\/jats:italic>\n K,in<\/jats:sub>\n by InsP\n 6<\/jats:sub>\n was reached much more quickly in\n Vicia<\/jats:italic>\n (3 min at \u22481 \u03bcM) than\n Solanum<\/jats:italic>\n (20\u201330 min). The effects of InsP\n 6<\/jats:sub>\n on\n I<\/jats:italic>\n K,in<\/jats:sub>\n were specific to the\n myo<\/jats:italic>\n -inositol isomer and were not elicited by other conformers of InsP\n 6<\/jats:sub>\n (e.g.,\n scyllo<\/jats:italic>\n - or\n neo<\/jats:italic>\n -). Chelation of Ca\n 2+<\/jats:sup>\n by inclusion of 1,2-bis(2-aminophenoxy)ethane-\n N,N,N<\/jats:italic>\n \u2032\n ,N<\/jats:italic>\n \u2032-tetraacetic acid or EGTA in the patch pipette together with InsP\n 6<\/jats:sub>\n prevented the inhibition of\n I<\/jats:italic>\n K,in<\/jats:sub>\n , suggesting that the effect is Ca\n 2+<\/jats:sup>\n dependent. InsP\n 6<\/jats:sub>\n was \u2248100-fold more potent than Ins(1,4,5)P\n 3<\/jats:sub>\n in modulating\n I<\/jats:italic>\n K,in<\/jats:sub>\n . Thus ABA increases InsP\n 6<\/jats:sub>\n in guard cells, and InsP\n 6<\/jats:sub>\n is a potent Ca\n 2+<\/jats:sup>\n -dependent inhibitor of\n I<\/jats:italic>\n K,in<\/jats:sub>\n . Taken together, these results suggest that InsP\n 6<\/jats:sub>\n may play a major role in the physiological response of guard cells to ABA.\n <\/jats:p>","DOI":"10.1073\/pnas.140217497","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:41:48Z","timestamp":1027680108000},"page":"8687-8692","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":167,"title":["Inositol hexakisphosphate is a physiological signal regulating the K\n +<\/sup>\n -inward rectifying conductance in guard cells"],"prefix":"10.1073","volume":"97","author":[{"given":"Fouad","family":"Lemtiri-Chlieh","sequence":"first","affiliation":[{"name":"Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enid A. C.","family":"MacRobbie","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles A.","family":"Brearley","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2000,7,11]]},"reference":[{"key":"e_1_3_3_1_2","volume-title":"Inositol Phosphates, Their Chemistry, Biochemistry and Physiology","author":"Cosgrove D J","year":"1980","unstructured":"D J Cosgrove Inositol Phosphates, Their Chemistry, Biochemistry and Physiology (Elsevier, Amsterdam, 1980)."},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3350671"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5424.96"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5460.2026"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5337.471"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01160403"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233456"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233479"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900114"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00195690"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/371711a0"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/346769a0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/346766a0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7732384"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1997.tb03052.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07238.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3140227"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3180279"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2540585"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.49.5.842"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/338427a0"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01258684"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.119.1.277"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004250050322"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00231883"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2760(98)00131-3"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.6.2202"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.122.4.1209"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.26.15837"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.3.987"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3240123"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.21.12192"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.4.1779"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.8.4778"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.080068897"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.140217497","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T23:06:14Z","timestamp":1649804774000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.140217497"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,7,11]]},"references-count":35,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2000,7,18]]}},"alternative-id":["10.1073\/pnas.140217497"],"URL":"https:\/\/doi.org\/10.1073\/pnas.140217497","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,7,11]]},"assertion":[{"value":"2000-05-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-07-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}