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Here, we show that many of these oligos additionally bind unintended mRNA targets as well, and that this effect overall dominates and complicates such screens. Focusing on the strong \u201coff-target\u201d signal, we design novel oligos that no longer bind any one gene specifically but nevertheless strongly and reproducibly block pathogen entry\u2014pointing to pathogen\/host interactions at a higher-order, pathway level.<\/jats:p>","DOI":"10.1073\/pnas.1402353111","type":"journal-article","created":{"date-parts":[[2014,3,10]],"date-time":"2014-03-10T21:34:20Z","timestamp":1394487260000},"page":"4548-4553","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":66,"title":["Specific inhibition of diverse pathogens in human cells by synthetic microRNA-like oligonucleotides inferred from RNAi screens"],"prefix":"10.1073","volume":"111","author":[{"given":"Andrea","family":"Franceschini","sequence":"first","affiliation":[{"name":"Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics, University of Zurich, CH-8057 Zurich, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Meier","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Eidgen\u00f6ssische Technische Hochschule Zurich, CH-8093 Zurich, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Casanova","sequence":"additional","affiliation":[{"name":"Biozentrum, University of Basel, CH-4056 Basel, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"Kreibich","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Eidgen\u00f6ssische Technische Hochschule Zurich, CH-8093 Zurich, Switzerland; 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