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Here, we describe, to our knowledge, the first molecular snapshot of bacterial ribosome recognition by a pathogen-derived, aminoglycoside-resistance rRNA methyltransferase. Our results support a model in which initial rigid docking on a highly conserved ribosome tertiary surface drives conformational changes in the enzyme that capture the target base within a remodeled active site. Extreme conservation of the ribosome-docking surface suggests there is no impediment to the spread of this resistance activity but also presents a target for specific inhibitor development.<\/jats:p>","DOI":"10.1073\/pnas.1402789111","type":"journal-article","created":{"date-parts":[[2014,4,9]],"date-time":"2014-04-09T21:59:16Z","timestamp":1397080756000},"page":"6275-6280","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":59,"title":["Molecular recognition and modification of the 30S ribosome by the aminoglycoside-resistance methyltransferase NpmA"],"prefix":"10.1073","volume":"111","author":[{"given":"Jack A.","family":"Dunkle","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kellie","family":"Vinal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja M.","family":"Desai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Zelinskaya","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miloje","family":"Savic","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayne M.","family":"West","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graeme L.","family":"Conn","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine M.","family":"Dunham","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,4,9]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/nrmicro3155","article-title":"Ribosome-targeting antibiotics and mechanisms of bacterial resistance","volume":"12","author":"Wilson DN","year":"2014","unstructured":"DN Wilson, Ribosome-targeting antibiotics and mechanisms of bacterial resistance. 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