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Here we report cloning of the gene and characterization of the gene product for a C\u22c5As lyase, ArsI, a member of the family of type I extradiol dioxygenases. ArsI is the only enzyme shown to be involved in degradation of the reduced forms of the herbicide monosodium methylarsonic acid and the antimicrobial growth promoter roxarsone. As\n                    <jats:italic>arsI<\/jats:italic>\n                    genes are widely distributed in bacteria, ArsI-catalyzed organoarsenic degradation is proposed to have an impact on the arsenic biogeocycle.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1403057111","type":"journal-article","created":{"date-parts":[[2014,5,13]],"date-time":"2014-05-13T04:53:58Z","timestamp":1399956838000},"page":"7701-7706","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":146,"title":["A C\u22c5As lyase for degradation of environmental organoarsenical herbicides and animal husbandry growth promoters"],"prefix":"10.1073","volume":"111","author":[{"given":"Masafumi","family":"Yoshinaga","sequence":"first","affiliation":[{"name":"Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33199"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry P.","family":"Rosen","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33199"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,5,12]]},"reference":[{"key":"e_1_3_4_1_2","article-title":"Earth abides arsenic biotransformations","author":"Zhu YG","year":"2014","unstructured":"YG Zhu, M Yoshinaga, FJ Zhao, BP Rosen, Earth abides arsenic biotransformations. 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Science 300, 939\u2013944 (2003).","journal-title":"Science"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"5213","DOI":"10.1073\/pnas.0900238106","article-title":"Biotransformation of arsenic by a Yellowstone thermoacidophilic eukaryotic alga","volume":"106","author":"Qin J","year":"2009","unstructured":"J Qin, et al., Biotransformation of arsenic by a Yellowstone thermoacidophilic eukaryotic alga. Proc Natl Acad Sci USA 106, 5213\u20135217 (2009).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1073\/pnas.0506836103","article-title":"Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S-adenosylmethionine methyltransferase","volume":"103","author":"Qin J","year":"2006","unstructured":"J Qin, et al., Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S-adenosylmethionine methyltransferase. Proc Natl Acad Sci USA 103, 2075\u20132080 (2006).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.tplants.2011.12.003","article-title":"Arsenic biomethylation by photosynthetic organisms","volume":"17","author":"Ye J","year":"2012","unstructured":"J Ye, C Rensing, BP Rosen, YG Zhu, Arsenic biomethylation by photosynthetic organisms. Trends Plant Sci 17, 155\u2013162 (2012).","journal-title":"Trends Plant Sci"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"379","DOI":"10.2134\/jeq2013.07.0268","article-title":"Arsenic retention in foliage and soil after monosodium methyl arsenate (MSMA) application to turfgrass","volume":"43","author":"Matteson AR","year":"2014","unstructured":"AR Matteson, et al., Arsenic retention in foliage and soil after monosodium methyl arsenate (MSMA) application to turfgrass. J Environ Qual 43, 379\u2013388 (2014).","journal-title":"J Environ Qual"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1021\/es026219q","article-title":"Environmental fate of roxarsone in poultry litter. I. Degradation of roxarsone during composting","volume":"37","author":"Garbarino JR","year":"2003","unstructured":"JR Garbarino, AJ Bednar, DW Rutherford, RS Beyer, RL Wershaw, Environmental fate of roxarsone in poultry litter. I. Degradation of roxarsone during composting. 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Environ Sci Technol 41, 818\u2013823 (2007).","journal-title":"Environ Sci Technol"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.2134\/jeq2007.0479","article-title":"Fate of arsenic in swine waste from concentrated animal feeding operations","volume":"37","author":"Makris KC","year":"2008","unstructured":"KC Makris, S Quazi, P Punamiya, D Sarkar, R Datta, Fate of arsenic in swine waste from concentrated animal feeding operations. J Environ Qual 37, 1626\u20131633 (2008).","journal-title":"J Environ Qual"},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1021\/jf60155a017","article-title":"Degradation of monosodium methanearsonic acid by soil microorganisms","volume":"16","author":"Von Endt DW","year":"1968","unstructured":"DW Von Endt, PC Kearney, DD Kafman, Degradation of monosodium methanearsonic acid by soil microorganisms. 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Environ Sci Technol 48, 1141\u20131147 (2014).","journal-title":"Environ Sci Technol"},{"key":"e_1_3_4_18_2","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/s10482-009-9313-z","article-title":"ArsR arsenic-resistance regulatory protein from Cupriavidus metallidurans CH34","volume":"96","author":"Zhang YB","year":"2009","unstructured":"YB Zhang, et al., ArsR arsenic-resistance regulatory protein from Cupriavidus metallidurans CH34. Antonie van Leeuwenhoek 96, 161\u2013170 (2009).","journal-title":"Antonie van Leeuwenhoek"},{"key":"e_1_3_4_19_2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1080\/10409230600817422","article-title":"The ins and outs of ring-cleaving dioxygenases","volume":"41","author":"Vaillancourt FH","year":"2006","unstructured":"FH Vaillancourt, JT Bolin, LD Eltis, The ins and outs of ring-cleaving dioxygenases. 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J Biol Chem 272, 15734\u201315738 (1997).","journal-title":"J Biol Chem"},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1107\/S1744309110027661","article-title":"Crystallization and preliminary X-ray crystallographic analysis of the ArsM arsenic(III) S-adenosylmethionine methyltransferase","volume":"66","author":"Marapakala K","year":"2010","unstructured":"K Marapakala, AA Ajees, J Qin, B Sankaran, BP Rosen, Crystallization and preliminary X-ray crystallographic analysis of the ArsM arsenic(III) S-adenosylmethionine methyltransferase. 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