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However, here we show that the antibiotic induces stalling, even without significant contacts with the peptide, by allosterically altering the peptidyl transferase center. This finding unveils a previously unknown role of cofactors for translation arrest and demonstrates the existence of a functional link between the exit tunnel and the catalytic center of the ribosome.<\/jats:p>","DOI":"10.1073\/pnas.1403586111","type":"journal-article","created":{"date-parts":[[2014,6,24]],"date-time":"2014-06-24T20:20:08Z","timestamp":1403641208000},"page":"9804-9809","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":117,"title":["Macrolide antibiotics allosterically predispose the ribosome for translation arrest"],"prefix":"10.1073","volume":"111","author":[{"given":"Shanmugapriya","family":"Sothiselvam","sequence":"first","affiliation":[{"name":"Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, IL 60607;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Liu","sequence":"additional","affiliation":[{"name":"Beckman Institute and Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Han","sequence":"additional","affiliation":[{"name":"Beckman Institute and Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haripriya","family":"Ramu","sequence":"additional","affiliation":[{"name":"Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, IL 60607;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Klepacki","sequence":"additional","affiliation":[{"name":"Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, IL 60607;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma Catherine","family":"Atkinson","sequence":"additional","affiliation":[{"name":"Institute of Technology, University of Tartu, 50411 Tartu, Estonia;"},{"name":"Department of Molecular Biology and"},{"name":"Laboratory for Molecular Infection Medicine Sweden, Ume\u00e5 University, Ume\u00e5 90187, Sweden; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Age","family":"Brauer","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology, University of Tartu, 50411 Tartu, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maido","family":"Remm","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology, University of Tartu, 50411 Tartu, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanel","family":"Tenson","sequence":"additional","affiliation":[{"name":"Institute of Technology, University of Tartu, 50411 Tartu, Estonia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Schulten","sequence":"additional","affiliation":[{"name":"Beckman Institute and Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"V\u00e1zquez-Laslop","sequence":"additional","affiliation":[{"name":"Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, IL 60607;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander S.","family":"Mankin","sequence":"additional","affiliation":[{"name":"Center for Pharmaceutical Biotechnology, University of Illinois, Chicago, IL 60607;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,6,24]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-4-431-55052-5_10"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-080211-105026"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.3.577"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06576.x"},{"key":"e_1_3_3_5_2","volume-title":"Antibiotic Drug Discovery and Development","author":"Subramanian SL","year":"2011","unstructured":"SL Subramanian, H Ramu, AS Mankin, Inducible resistance to macrolide antibiotics. 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