{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T10:08:27Z","timestamp":1792490907325,"version":"4.5.0"},"reference-count":37,"publisher":"National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2014,4,22]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Blood platelets, the small circulating cells that coordinate hemostasis, are produced by specialized bone marrow cells called megakaryocytes. The cytokine thrombopoietin (TPO) is a key regulator of platelet production acting via its specific cell receptor, Mpl. Via genetic modification of the\n                    <jats:italic>Mpl<\/jats:italic>\n                    allele in mice, we precisely define the bone marrow cells that express Mpl and, by genetically removing Mpl from megakaryocytes and platelets, we show TPO signaling via Mpl is not required in megakaryocytes for their expansion, maturation, or platelet production. Rather, Mpl expression on megakaryocytes is essential for regulating TPO availability in the bone marrow microenvironment to prevent myeloproliferation, a model we suggest is important for human disease.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1404354111","type":"journal-article","created":{"date-parts":[[2014,4,8]],"date-time":"2014-04-08T00:10:32Z","timestamp":1396915832000},"page":"5884-5889","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":137,"title":["Mpl expression on megakaryocytes and platelets is dispensable for thrombopoiesis but essential to prevent myeloproliferation"],"prefix":"10.1073","volume":"111","author":[{"given":"Ashley P.","family":"Ng","sequence":"first","affiliation":[{"name":"Divisions of aCancer and Haematology, and"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Kauppi","sequence":"additional","affiliation":[{"name":"Divisions of aCancer and Haematology, and"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald","family":"Metcalf","sequence":"additional","affiliation":[{"name":"Divisions of aCancer and Haematology, and"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig D.","family":"Hyland","sequence":"additional","affiliation":[{"name":"Divisions of aCancer and Haematology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma C.","family":"Josefsson","sequence":"additional","affiliation":[{"name":"Divisions of aCancer and Haematology, and"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Lebois","sequence":"additional","affiliation":[{"name":"Divisions of aCancer and Haematology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Guo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Divisions of aCancer and Haematology, and"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracey M.","family":"Baldwin","sequence":"additional","affiliation":[{"name":"Molecular Medicine, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ladina","family":"Di Rago","sequence":"additional","affiliation":[{"name":"Divisions of aCancer and Haematology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas J.","family":"Hilton","sequence":"additional","affiliation":[{"name":"Molecular Medicine, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia; and"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warren S.","family":"Alexander","sequence":"additional","affiliation":[{"name":"Divisions of aCancer and Haematology, and"},{"name":"Department of Medical Biology, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,4,7]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/B978-0-12-387837-3.00059-6","volume-title":"Platelets","author":"Kuter DJ","year":"2013","unstructured":"DJ Kuter, Thrombopoietin Mimetics. 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