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We have used a cell-surface fluorescence assay to reveal novel interactions among CMV glycoproteins and probe the effect of MSL-109 on glycoprotein interactions. Our data confirm cross-talk between gH\/gL and gB, the fusion protein. Moreover, we identify a homophilic interaction between gH\/gL heterodimers that is perturbed by MSL-109. This study suggests a novel mechanism of neutralization, and highlights potential strategies for treatment of CMV disease.<\/jats:p>","DOI":"10.1073\/pnas.1404653111","type":"journal-article","created":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T22:36:03Z","timestamp":1400538963000},"page":"8209-8214","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":61,"title":["Mechanism for neutralizing activity by the anti-CMV gH\/gL monoclonal antibody MSL-109"],"prefix":"10.1073","volume":"111","author":[{"given":"Ashley E.","family":"Fouts","sequence":"first","affiliation":[{"name":"Departments of aInfectious Diseases,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"La\u00ebtitia","family":"Comps-Agrar","sequence":"additional","affiliation":[{"name":"Protein Chemistry,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina F.","family":"Stengel","sequence":"additional","affiliation":[{"name":"Structural Biology,"},{"name":"Early Discovery Biochemistry, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Ellerman","sequence":"additional","affiliation":[{"name":"Protein Chemistry,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allyn J.","family":"Schoeffler","sequence":"additional","affiliation":[{"name":"Early Discovery Biochemistry, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren","family":"Warming","sequence":"additional","affiliation":[{"name":"Molecular Biology, Genentech Inc., South San Francisco, CA 94080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan L.","family":"Eaton","sequence":"additional","affiliation":[{"name":"Protein Chemistry,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Becket","family":"Feierbach","sequence":"additional","affiliation":[{"name":"Departments of aInfectious Diseases,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,5,19]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"S839","DOI":"10.1093\/clinids\/12.Supplement_7.S839","article-title":"Cytomegalovirus immunoglobulins in the prevention and treatment of cytomegalovirus disease","volume":"12","author":"Snydman DR","year":"1990","unstructured":"DR Snydman, Cytomegalovirus immunoglobulins in the prevention and treatment of cytomegalovirus disease. 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