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Mutagenesis targeted to unblock the steric hindrance in the active pocket or the potential product release site resulted in variants with much higher activity toward \u03b1-naphthyl glycidyl ether, the precursor of \u03b2-adrenergic receptor blocking drug (\n                    <jats:italic>S<\/jats:italic>\n                    )-propranolol. The strategy presented here may be a useful alternative choice for rational design of enzymes toward bulky substrates.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1404915111","type":"journal-article","created":{"date-parts":[[2014,10,21]],"date-time":"2014-10-21T02:45:44Z","timestamp":1413859544000},"page":"15717-15722","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":93,"title":["Engineering of an epoxide hydrolase for efficient bioresolution of bulky pharmaco substrates"],"prefix":"10.1073","volume":"111","author":[{"given":"Xu-Dong","family":"Kong","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;"},{"name":"State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuguang","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"She","family":"Chen","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Beijing 102206, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-He","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahai","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,10,20]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/S1367-5931(00)00179-4","article-title":"Synthetic applications of epoxide hydrolases","volume":"5","author":"Archelas A","year":"2001","unstructured":"A Archelas, R Furstoss, Synthetic applications of epoxide hydrolases. 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