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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,4,21]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Infiltration of immune cells into adipose tissue has been observed in obesity, but, until now, these data were based on immunohistological microscopic analysis. Here, to our knowledge for the first time, we applied an intravital multiphoton imaging technique to adipose tissue with lysozyme M-EGFP transgenic (LysM\n                    <jats:sup>EGFP<\/jats:sup>\n                    ) mice whose EGFP was expressed in the myelomonocytic lineage. Mobility of LysM\n                    <jats:sup>EGFP<\/jats:sup>\n                    -positive macrophages was activated just 5 d after high-fat and high-sucrose (HF\/HS) diet before the development of obesity. Furthermore, a significant increase of S100A8, one of the alarmins, was detected in fat tissue just 5 d after HF\/HS diet. S100A8 stimulated chemotactic migration, and neutralization of S100A8 suppressed the HF\/HS diet-induced activation of LysM\n                    <jats:sup>EGFP<\/jats:sup>\n                    -positive cells. Time-lapse intravital imaging first identified the very early event exhibiting increased mobility of adipose macrophages.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1409480112","type":"journal-article","created":{"date-parts":[[2015,4,6]],"date-time":"2015-04-06T22:23:05Z","timestamp":1428358985000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":47,"title":["Visualized macrophage dynamics and significance of S100A8 in obese fat"],"prefix":"10.1073","volume":"112","author":[{"given":"Ryohei","family":"Sekimoto","sequence":"first","affiliation":[{"name":"Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norikazu","family":"Maeda","sequence":"additional","affiliation":[{"name":"Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Tsushima","sequence":"additional","affiliation":[{"name":"Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Nakatsuji","sequence":"additional","affiliation":[{"name":"Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Nishizawa","sequence":"additional","affiliation":[{"name":"Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Kishida","sequence":"additional","affiliation":[{"name":"Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Kikuta","sequence":"additional","affiliation":[{"name":"Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;"},{"name":"Laboratory of Cellular Dynamics, World Premier International Immunology Frontier Research Center, Osaka University, Osaka 565-0871, Japan;"},{"name":"Japan Science and Technology, Core Research for Evolutionary Science and Technology, Tokyo 102-0075, Japan; 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