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Here we demonstrate that cells from the canal of Schlemm of glaucomatous eyes have altered gene expression and increased cytoskeletal stiffness that leads to reduced pore formation in these cells, likely accounting for increased outflow resistance associated with glaucoma. These findings thus establish that dysfunctional cytoskeletal mechanics may lie at the heart of this disease process and thereby motivate development of glaucoma therapeutics that target cell stiffness.<\/jats:p>","DOI":"10.1073\/pnas.1410602111","type":"journal-article","created":{"date-parts":[[2014,9,9]],"date-time":"2014-09-09T00:38:33Z","timestamp":1410223113000},"page":"13876-13881","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":176,"title":["Altered mechanobiology of Schlemm\u2019s canal endothelial cells in glaucoma"],"prefix":"10.1073","volume":"111","author":[{"given":"Darryl R.","family":"Overby","sequence":"first","affiliation":[{"name":"Department of Bioengineering, Imperial College London, London SW7 2AZ, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enhua H.","family":"Zhou","sequence":"additional","affiliation":[{"name":"Program in Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rocio","family":"Vargas-Pinto","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan M.","family":"Pedrigi","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Imperial College London, London SW7 2AZ, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Fuchshofer","sequence":"additional","affiliation":[{"name":"Institute of Human Anatomy and Embryology, Universit\u00e4t Regensburg, 93053 Regensburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sietse T.","family":"Braakman","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Imperial College London, London SW7 2AZ, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritika","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Imperial College London, London SW7 2AZ, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin M.","family":"Perkumas","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Duke University, Durham, NC 27710;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph M.","family":"Sherwood","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Imperial College London, London SW7 2AZ, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Vahabikashi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quynh","family":"Dang","sequence":"additional","affiliation":[{"name":"Program in Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Hun","family":"Kim","sequence":"additional","affiliation":[{"name":"Program in Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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