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This study demonstrates the clearance of latent Epstein\u2013Barr virus genomes in a subpopulation of Burkitt\u2019s lymphoma patient-derived cells with clustered regularly interspaced short palindromic repeat\/Cas9 nuclease. Viral genome destruction leads to proliferation arrest and apoptosis in Epstein\u2013Barr virus-infected cells, with no observed cytotoxicity to noninfected cells. Although many hurdles remain before this approach could be used in the clinic, this strategy may lead to a generalized approach to cure latent viral infections.<\/jats:p>","DOI":"10.1073\/pnas.1410785111","type":"journal-article","created":{"date-parts":[[2014,8,25]],"date-time":"2014-08-25T23:20:35Z","timestamp":1409008835000},"page":"13157-13162","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":199,"title":["RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection"],"prefix":"10.1073","volume":"111","author":[{"given":"Jianbin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Bioengineering,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen R.","family":"Quake","sequence":"additional","affiliation":[{"name":"Department of Bioengineering,"},{"name":"Department of Applied Physics, and"},{"name":"Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,8,25]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511545313.054"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/338231"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4682-8-23"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt571"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/mtna.2013.75"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1179555"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.03.005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-110410-132430"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10886"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1225829"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1231143"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.00471"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1232033"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.022"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.06.044"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.08.022"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.04.025"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1246981"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247005"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13166"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(64)91524-7"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(64)92345-1"},{"key":"e_1_3_3_23_2","first-page":"547","article-title":"Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji)","volume":"37","author":"Epstein MA","year":"1966","unstructured":"MA Epstein, et al., Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji). 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