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In addition, the CPD viruses exhibited a range of restriction in mice and African green monkeys that compared favorably with existing attenuated strains presently in clinical studies. This study produced examples of a new type of vaccine candidate for RSV and showed that CPD of a nonsegmented negative-strand RNA virus can rapidly generate vaccine candidates with a range of attenuation.<\/jats:p>","DOI":"10.1073\/pnas.1411290111","type":"journal-article","created":{"date-parts":[[2014,8,25]],"date-time":"2014-08-25T23:20:35Z","timestamp":1409008835000},"page":"13169-13174","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":133,"title":["Attenuation of human respiratory syncytial virus by genome-scale codon-pair deoptimization"],"prefix":"10.1073","volume":"111","author":[{"given":"Cyril","family":"Le Nou\u00ebn","sequence":"first","affiliation":[{"name":"RNA Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda G.","family":"Brock","sequence":"additional","affiliation":[{"name":"RNA Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy","family":"Luongo","sequence":"additional","affiliation":[{"name":"RNA Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"McCarty","sequence":"additional","affiliation":[{"name":"RNA Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Yang","sequence":"additional","affiliation":[{"name":"RNA Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masfique","family":"Mehedi","sequence":"additional","affiliation":[{"name":"RNA Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eckard","family":"Wimmer","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Mueller","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter L.","family":"Collins","sequence":"additional","affiliation":[{"name":"RNA Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula J.","family":"Buchholz","sequence":"additional","affiliation":[{"name":"RNA Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua M.","family":"DiNapoli","sequence":"additional","affiliation":[{"name":"RNA Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,8,25]]},"reference":[{"key":"e_1_3_4_1_2","unstructured":"PL Collins RA Karron Respiratory syncytial virus and metapneumovirus. 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