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This step is followed by binding of 50S ribosome and release of IFs to form elongation competent 70S complex. tRNA\n                    <jats:sup>fMet<\/jats:sup>\n                    is special in possessing a vital feature of three consecutive G-C (3GC) base pairs in its anticodon stem. However, the role of this feature has remained unclear. We show that the 3GC base pairs facilitate tRNA\n                    <jats:sup>fMet<\/jats:sup>\n                    retention in the ribosome during the transitions that mark conversion of 30S PIC into 70S complex. Furthermore, we show that translation of mRNAs having an extended Shine\u2013Dalgarno sequence bypasses the requirement of the 3GC pairs in tRNA\n                    <jats:sup>fMet<\/jats:sup>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.1411637111","type":"journal-article","created":{"date-parts":[[2014,9,23]],"date-time":"2014-09-23T03:21:36Z","timestamp":1411442496000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":13,"title":["An extended Shine\u2013Dalgarno sequence in mRNA functionally bypasses a vital defect in initiator tRNA"],"prefix":"10.1073","volume":"111","author":[{"given":"Sunil","family":"Shetty","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HimaPriyanka","family":"Nadimpalli","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riyaz Ahmad","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smriti","family":"Arora","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gautam","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umesh","family":"Varshney","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India; and"},{"name":"Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560064, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,9,22]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.57.070188.001215"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.4.1342"},{"key":"e_1_3_3_3_2","first-page":"473","article-title":"Role of the three consecutive G:C base pairs conserved in the anticodon stem of initiator tRNAs in initiation of protein synthesis in Escherichia coli","volume":"2","author":"Mandal N","year":"1996","unstructured":"N Mandal, D Mangroo, JJ Dalluge, JA McCloskey, UL Rajbhandary, Role of the three consecutive G:C base pairs conserved in the anticodon stem of initiator tRNAs in initiation of protein synthesis in Escherichia coli. 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