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Here, we perform experiments in the kidney from Kcnj10 knockout mice and demonstrate that Kcnj10 is a main contributor to the basolateral potassium conductance in the DCT and that the potassium channel activity regulates the expression of ste20-related proline\u2013alanine-rich kinase (SPAK) and determines Na-Cl cotransporter (NCC) expression. Our study suggests the possibility that the regulation of basolateral Kcnj10 activity in the DCT may be the first step in response to a variety of physiological stimuli for initiating SPAK-WNK-dependent modulation of NCC expression in the kidney.<\/jats:p>","DOI":"10.1073\/pnas.1411705111","type":"journal-article","created":{"date-parts":[[2014,7,29]],"date-time":"2014-07-29T02:39:50Z","timestamp":1406601590000},"page":"11864-11869","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":158,"title":["KCNJ10 determines the expression of the apical Na-Cl cotransporter (NCC) in the early distal convoluted tubule (DCT1)"],"prefix":"10.1073","volume":"111","author":[{"given":"Chengbiao","family":"Zhang","sequence":"first","affiliation":[{"name":"Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221002, China;"},{"name":"Department of Pharmacology, New York Medical College, Valhalla, NY 10595; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, New York Medical College, Valhalla, NY 10595; 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