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The structure of heterodimeric\n                    <jats:italic>Thermus thermophilus<\/jats:italic>\n                    phenylalanyl-tRNA synthetase (\n                    <jats:italic>Tt<\/jats:italic>\n                    PheRS) with the aminonucleoside antibiotic puromycin reveals the detailed architecture of the complex with ligand mimicking the A76 of tRNA misacylated with noncognate Tyr. Based on the crystal structure and using quantum mechanics\/molecular mechanics approach, we present a universal hydrolytic mechanism utilizing cyclic 2\u2032,3\u2032-intermediates for\n                    <jats:italic>Tt<\/jats:italic>\n                    PheRS, and potentially for other class I and class II aaRSs.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1414852112","type":"journal-article","created":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T22:56:33Z","timestamp":1426546593000},"page":"3967-3972","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":12,"title":["Universal pathway for posttransfer editing reactions: Insights from the crystal structure of\n                    <i>Tt<\/i>\n                    PheRS with puromycin"],"prefix":"10.1073","volume":"112","author":[{"given":"Dmitry","family":"Tworowski","sequence":"first","affiliation":[{"name":"Department of Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liron","family":"Klipcan","sequence":"additional","affiliation":[{"name":"Medical Research Council, Mitochondrial Biology Unit, Cambridge CB2 0XY, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moshe","family":"Peretz","sequence":"additional","affiliation":[{"name":"Department of Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Moor","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark G.","family":"Safro","sequence":"additional","affiliation":[{"name":"Department of Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,3,16]]},"reference":[{"key":"e_1_3_4_1_2","first-page":"1","article-title":"Synthetic and editing mechanisms of aminoacyl-tRNA synthetases","volume":"344","author":"Perona JJ","year":"2014","unstructured":"JJ Perona, I Gruic-Sovulj, Synthetic and editing mechanisms of aminoacyl-tRNA synthetases. 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