{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T11:54:27Z","timestamp":1793015667899,"version":"4.5.0"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"51","license":[{"start":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T00:00:00Z","timestamp":1435017600000},"content-version":"vor","delay-in-days":197,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/501100001505","name":"Health Research Council of New Zealand (HRC)","doi-asserted-by":"crossref","award":["12\/670"],"award-info":[{"award-number":["12\/670"]}],"id":[{"id":"10.13039\/501100001505","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2014,12,23]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>The mammalian reproductive system is critically dependent upon pulsatile hormone release patterned and driven by a small population of gonadotropin-releasing hormone (GnRH) neurons. The scattered distribution of GnRH neurons within the brain has made it extremely difficult to investigate the nature and properties of these cells in any selective manner in vivo. We report here a procedure that enables selective, high-fidelity activation of the GnRH neurons that control gonadotropin secretion in ovariectomized mice. Using this approach, we have been able to define minimal parameters of activation required to generate pulsatile gonadotropin secretion in the blood. This provides critical information for understanding and manipulating the genesis of gonadotropin pulsatility in reproductive biology.<\/jats:p>","DOI":"10.1073\/pnas.1415226112","type":"journal-article","created":{"date-parts":[[2014,12,9]],"date-time":"2014-12-09T00:58:08Z","timestamp":1418086688000},"page":"18387-18392","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":85,"title":["Optogenetic activation of GnRH neurons reveals minimal requirements for pulsatile luteinizing hormone secretion"],"prefix":"10.1073","volume":"111","author":[{"given":"Pauline","family":"Campos","sequence":"first","affiliation":[{"name":"Centre for Neuroendocrinology and Department of Physiology, Otago School of Medical Sciences, University of Otago, Dunedin 9054, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan E.","family":"Herbison","sequence":"additional","affiliation":[{"name":"Centre for Neuroendocrinology and Department of Physiology, Otago School of Medical Sciences, University of Otago, Dunedin 9054, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2014,12,8]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.100883"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-111-5-1737"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-111-5-1449"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-122-5-2211"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0820263"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-123-4-1808"},{"key":"e_1_3_3_7_2","first-page":"399","volume-title":"Knobil and Neill\u2019s Physiology of Reproduction","author":"Herbison AE","year":"2014","unstructured":"AE Herbison, Physiology of the adult GnRH neuronal network. Knobil and Neill\u2019s Physiology of Reproduction, eds TM Plant, AJ Zeleznik (Academic Press, San Diego), pp. 399\u2013467 (2014)."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.130.1.1727719"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-3022(03)00013-X"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0533-13.2013"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-1711"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-14-05898.1999"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1618-13.2013"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2013-1502"},{"key":"e_1_3_3_15_2","first-page":"2312","article-title":"Localization of luteinizing hormone-releasing hormone (LHRH) neurons that project to the median eminence","volume":"7","author":"Silverman AJ","year":"1987","unstructured":"AJ Silverman, J Jhamandas, LP Renaud, Localization of luteinizing hormone-releasing hormone (LHRH) neurons that project to the median eminence. J Neurosci 7, 2312\u20132319 (1987).","journal-title":"J Neurosci"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5759-10.2011"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.03.055"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00239310"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1631"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6156-09.2010"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.10.7690"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-06-02313.2002"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3171-06.2006"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2010.05.005"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2962-09.2009"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2826.1997.00615.x"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0579-13.2013"},{"key":"e_1_3_3_28_2","article-title":"Multitasking in GnRH neuron dendrites","author":"Iremonger KJ","year":"2014","unstructured":"KJ Iremonger, AE Herbison, Multitasking in GnRH neuron dendrites. Neuroendocrinology, , in press. (2014).","journal-title":"Neuroendocrinology"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.06.004"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1159\/000124988"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1139"},{"key":"e_1_3_3_32_2","volume-title":"The Mouse Brain in Stereotaxic Coordinates","author":"Paxinos G","year":"2004","unstructured":"G Paxinos, KBJ Franklin The Mouse Brain in Stereotaxic Coordinates (Academic Press, San Diego, 2004)."},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039001"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011-1228"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1415226112","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1415226112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:35:30Z","timestamp":1649810130000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1415226112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,8]]},"references-count":34,"journal-issue":{"issue":"51","published-print":{"date-parts":[[2014,12,23]]}},"alternative-id":["10.1073\/pnas.1415226112"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1415226112","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,8]]},"assertion":[{"value":"2014-12-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}