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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,3,3]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ions serve as a key cellular signal and are tightly controlled. One mechanism to limit free Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ions is buffering by Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -binding proteins, which are strongly expressed in sensory hair cells of the ear. Here we studied how genetic disruption of the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -binding proteins parvalbumin-\u03b1, calbindin-D28k, and calretinin affects exocytosis and sound encoding at the synapses of mouse inner hair cells (IHCs) and spiral ganglion neurons (SGNs). Mutant IHCs showed increased exocytosis, but the sound-evoked spiking activity in SGNs was unaltered. Together with mathematical modeling, this finding indicates that a large fraction of exocytosis in mutant IHCs occurred outside synapses. We conclude that Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -binding proteins shape presynaptic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signals to restrict exocytosis to active zones, thus enabling metabolically efficient sound encoding.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1416424112","type":"journal-article","created":{"date-parts":[[2015,2,17]],"date-time":"2015-02-17T23:44:40Z","timestamp":1424216680000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":114,"title":["EF-hand protein Ca\n                    <sup>2+<\/sup>\n                    buffers regulate Ca\n                    <sup>2+<\/sup>\n                    influx and exocytosis in sensory hair cells"],"prefix":"10.1073","volume":"112","author":[{"given":"Tina","family":"Pangr\u0161i\u010d","sequence":"first","affiliation":[{"name":"Institute for Auditory Neuroscience and InnerEarLab, University Medical Center G\u00f6ttingen, 37099 G\u00f6ttingen, Germany;"},{"name":"Collaborative Research Center 889, University of G\u00f6ttingen, 37099 G\u00f6ttingen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mantas","family":"Gabrielaitis","sequence":"additional","affiliation":[{"name":"Institute for Auditory Neuroscience and InnerEarLab, University Medical Center G\u00f6ttingen, 37099 G\u00f6ttingen, Germany;"},{"name":"Theoretical Neurophysics Group, Max Planck Institute for Dynamics and Self-Organization, 37077 G\u00f6ttingen, Germany;"},{"name":"Bernstein Center for Computational Neuroscience, G\u00f6ttingen University, 37077 G\u00f6ttingen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susann","family":"Michanski","sequence":"additional","affiliation":[{"name":"Institute for Auditory Neuroscience and InnerEarLab, University Medical Center G\u00f6ttingen, 37099 G\u00f6ttingen, Germany;"},{"name":"Collaborative Research Center 889, University of G\u00f6ttingen, 37099 G\u00f6ttingen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beat","family":"Schwaller","sequence":"additional","affiliation":[{"name":"Unit of Anatomy, Department of Medicine, University of Fribourg, 1700 Fribourg, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred","family":"Wolf","sequence":"additional","affiliation":[{"name":"Collaborative Research Center 889, University of G\u00f6ttingen, 37099 G\u00f6ttingen, Germany;"},{"name":"Theoretical Neurophysics Group, Max Planck Institute for Dynamics and Self-Organization, 37077 G\u00f6ttingen, Germany;"},{"name":"Bernstein Center for Computational Neuroscience, G\u00f6ttingen University, 37077 G\u00f6ttingen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Strenzke","sequence":"additional","affiliation":[{"name":"Collaborative Research Center 889, University of G\u00f6ttingen, 37099 G\u00f6ttingen, Germany;"},{"name":"Auditory Systems Physiology Group, InnerEarLab, Department of Otolaryngology, University Medical Center G\u00f6ttingen, 37099 G\u00f6ttingen, Germany; and"},{"name":"Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Harvard University, Boston, MA 02114"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Moser","sequence":"additional","affiliation":[{"name":"Institute for Auditory Neuroscience and InnerEarLab, University Medical Center G\u00f6ttingen, 37099 G\u00f6ttingen, Germany;"},{"name":"Collaborative Research Center 889, University of G\u00f6ttingen, 37099 G\u00f6ttingen, Germany;"},{"name":"Bernstein Center for Computational Neuroscience, G\u00f6ttingen University, 37077 G\u00f6ttingen, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,2,17]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0959-4388(94)90049-3"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-05-03246.1994"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/147342202320883551"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2005.05.006"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10162-002-2050-x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/363074a0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/77687"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1196-05.2005"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0785-08.2008"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2005.04275.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.05046.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2007.01.002"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00635.2009"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2011.11.008"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-11-04577.2003"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00013-1"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-34-10832.2003"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10162-003-4020-3"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03418"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-18-06961.1997"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(00)76537-4"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.2.883"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00574.2004"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0389-10.2010"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3491-12.2013"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1978.41.2.365"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.10.027"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2006.06.008"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2012.09.002"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2578"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5453-10.2011"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1121\/1.393530"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(93)90103-8"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)01025-5"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23345"},{"key":"e_1_3_3_36_2","first-page":"247","article-title":"Developmental refinement of hair cell synapses tightens the coupling of Ca2+ influx to exocytosis","volume":"33","author":"Wong AB","year":"2014","unstructured":"AB Wong, , Developmental refinement of hair cell synapses tightens the coupling of Ca2+ influx to exocytosis. 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