{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T21:54:50Z","timestamp":1789854890763,"version":"4.0.1"},"reference-count":47,"publisher":"National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,3,17]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Malaria caused by\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    is a severe infectious disease with high mortality and morbidity rates worldwide. Chloroquine (CQ) is a widely used antimalarial agent, but the emergence and spread of CQ-resistant parasites is a growing global health problem. Although its physiological relevance remains unknown,\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    CQ resistance transporter (PfCRT) confers CQ resistance through CQ egress from digestive vacuoles of\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    . To address this issue, recombinant CQ-sensitive or CQ-resistant PfCRT proteins were purified and their transport activities were assessed. Both CQ-sensitive and CQ-resistant PfCRTs transported CQ, various antimalarial agents, and basic amino acids, indicating that PfCRT is a polyspecific drug and nutrient exporter.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1417102112","type":"journal-article","created":{"date-parts":[[2015,3,2]],"date-time":"2015-03-02T22:13:24Z","timestamp":1425334404000},"page":"3356-3361","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":71,"title":["<i>Plasmodium falciparum<\/i>\n                    chloroquine resistance transporter is a H\n                    <sup>+<\/sup>\n                    -coupled polyspecific nutrient and drug exporter"],"prefix":"10.1073","volume":"112","author":[{"given":"Narinobu","family":"Juge","sequence":"first","affiliation":[{"name":"Advanced Science Research Center,"},{"name":"Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Tokyo 113-8656, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sawako","family":"Moriyama","sequence":"additional","affiliation":[{"name":"Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaaki","family":"Miyaji","sequence":"additional","affiliation":[{"name":"Advanced Science Research Center,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamiyo","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruka","family":"Iwai","sequence":"additional","affiliation":[{"name":"Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoya","family":"Fukui","sequence":"additional","affiliation":[{"name":"Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, the George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Omote","sequence":"additional","affiliation":[{"name":"Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Moriyama","sequence":"additional","affiliation":[{"name":"Advanced Science Research Center,"},{"name":"Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,3,2]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01043-6"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2012.08.002"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(02)02249-3"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.77.181"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI38307"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2972"},{"key":"e_1_3_3_7_2","volume-title":"WHO Global Malaria Program World Malaria Report","author":"World Health Organization","year":"2011","unstructured":"; World Health Organization WHO Global Malaria Program World Malaria Report (WHO, Geneva, 2011)."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(05)00077-8"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2006.09.005"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.15"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi101638n"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07855.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msh205"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26818-0"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M503227200"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1175667"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.8.3382"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80447-X"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074045"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07366.x"},{"key":"e_1_3_3_21_2","first-page":"275","article-title":"Hemoglobin degradation","volume":"295","author":"Goldberg DE","year":"2005","unstructured":"DE Goldberg, Hemoglobin degradation. Curr Top Microbiol Immunol 295, 275\u2013291 (2005).","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2007.12.004"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20131007"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003323200"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3470243"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208648200"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05368.x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.125492"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00033.2012"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.09.002"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00040.2003"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2006.09.001"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200101253440403"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000887"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2004.03.016"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.11.3500-3505.2003"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002484"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039569"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04035.x"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05511.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06108.x"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600681"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.016758"},{"key":"e_1_3_3_44_2","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1111\/j.1365-2958.2010.07272.x","article-title":"Efflux of a range of antimalarial drugs and \u2018chloroquine resistance reversers\u2019 from the digestive vacuole in malaria parasites with mutant PfCRT","volume":"77","author":"Lehane AM","year":"2010","unstructured":"AM Lehane, K Kirk, Efflux of a range of antimalarial drugs and \u2018chloroquine resistance reversers\u2019 from the digestive vacuole in malaria parasites with mutant PfCRT. Mol Microbiol 77, 1039\u20131051 (2010).","journal-title":"Mol Microbiol"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.2174\/138945006778019372"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.09.012"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb4008953"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1417102112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T11:22:44Z","timestamp":1717759364000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1417102112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,2]]},"references-count":47,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,3,17]]}},"alternative-id":["10.1073\/pnas.1417102112"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1417102112","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,2]]},"assertion":[{"value":"2015-03-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}