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Here, we show that prolonged treatment of mouse insulinoma 6 (MIN6) cells and mouse pancreatic islets with\n                    <jats:sc>l<\/jats:sc>\n                    -cysteine inhibited glucose-stimulated insulin secretion (GSIS).\n                    <jats:sc>l<\/jats:sc>\n                    -cysteine reversibly inactivated pyruvate kinase muscle isoform 2 (PKM2), an isoform of the rate-limiting enzyme pyruvate kinase in glycolysis, by inducing PKM2 subunit dissociation (tetramers to dimers\/monomers), which resulted in impairment of glucose-induced ATP production and subsequent GSIS. Our findings in this work will raise caution about using a variety of\n                    <jats:sc>l<\/jats:sc>\n                    -cysteine containing supplements to diabetic patients and shed a light on a previously unidentified physiological role of\n                    <jats:sc>l<\/jats:sc>\n                    -cysteine and PKM2.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1417197112","type":"journal-article","created":{"date-parts":[[2015,2,25]],"date-time":"2015-02-25T17:27:01Z","timestamp":1424885221000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":65,"title":["<scp>l<\/scp>\n                    -cysteine reversibly inhibits glucose-induced biphasic insulin secretion and ATP production by inactivating PKM2"],"prefix":"10.1073","volume":"112","author":[{"given":"Daiki","family":"Nakatsu","sequence":"first","affiliation":[{"name":"Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuta","family":"Horiuchi","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumi","family":"Kano","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan;"},{"name":"Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taichi","family":"Sugawara","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iseki","family":"Takamoto","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoto","family":"Kubota","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan;"},{"name":"Department of Clinical Nutrition Therapy, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan; 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