{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:13:11Z","timestamp":1789863191127,"version":"4.0.1"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"48","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2014,12,2]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Serogroup B meningococcus (MenB) causes severe sepsis and invasive meningococcal disease, particularly affecting young children and adolescents. The genome-derived vaccine 4CMenB that targets MenB, has now been approved in over 30 countries worldwide. Here we report the crystal structure of the trimeric autotransporter Neisserial adhesin A (NadA), one of the three protein antigens included in 4CMenB, and the epitope mapping of a bactericidal mAb monoclonal antibody that targets the functional head domain of NadA. These results provide important insights into the structure and vaccine-induced immune response of this meningococcal antigen and may inform the engineering of improved immunogens by structure-based design.<\/jats:p>","DOI":"10.1073\/pnas.1419686111","type":"journal-article","created":{"date-parts":[[2014,11,20]],"date-time":"2014-11-20T03:43:24Z","timestamp":1416455004000},"page":"17128-17133","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":64,"title":["Structure of the meningococcal vaccine antigen NadA and epitope mapping of a bactericidal antibody"],"prefix":"10.1073","volume":"111","author":[{"given":"Enrico","family":"Malito","sequence":"first","affiliation":[{"name":"Novartis Vaccines, 53100 Siena, Italy; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Biancucci","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, 53100 Siena, Italy; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnese","family":"Faleri","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, 53100 Siena, Italy; 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Vaccine 30, B3\u2013B9 (2012).","journal-title":"Vaccine"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"B51","DOI":"10.1016\/j.vaccine.2009.04.063","article-title":"Global epidemiology of meningococcal disease","volume":"27","author":"Harrison LH","year":"2009","unstructured":"LH Harrison, CL Trotter, ME Ramsay, Global epidemiology of meningococcal disease. Vaccine 27, B51\u2013B63 (2009).","journal-title":"Vaccine"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"B30","DOI":"10.1016\/j.vaccine.2009.05.003","article-title":"Quadrivalent meningococcal conjugate vaccines","volume":"27","author":"Pace D","year":"2009","unstructured":"D Pace, AJ Pollard, NE Messonier, Quadrivalent meningococcal conjugate vaccines. Vaccine 27, B30\u2013B41 (2009).","journal-title":"Vaccine"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1056\/NEJM200105033441807","article-title":"Meningococcal disease","volume":"344","author":"Rosenstein NE","year":"2001","unstructured":"NE Rosenstein, BA Perkins, DS Stephens, T Popovic, JM Hughes, Meningococcal disease. N Engl J Med 344, 1378\u20131388 (2001).","journal-title":"N Engl J Med"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"123ps125","DOI":"10.1126\/scitranslmed.3003859","article-title":"Toward a meningitis-free world","volume":"4","author":"Black S","year":"2012","unstructured":"S Black, M Pizza, M Nissum, R Rappuoli, Toward a meningitis-free world. 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Vaccine 26, 4244\u20134250 (2008).","journal-title":"Vaccine"},{"key":"e_1_3_4_18_2","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1086\/656741","article-title":"Multicenter, open-label, randomized phase II controlled trial of an investigational recombinant Meningococcal serogroup B vaccine with and without outer membrane vesicles, administered in infancy","volume":"51","author":"Findlow J","year":"2010","unstructured":"J Findlow, , Multicenter, open-label, randomized phase II controlled trial of an investigational recombinant Meningococcal serogroup B vaccine with and without outer membrane vesicles, administered in infancy. 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J Bacteriol 193, 107\u2013115 (2011).","journal-title":"J Bacteriol"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","first-page":"20536","DOI":"10.1074\/jbc.M110.188326","article-title":"Neisseria meningitidis adhesin NadA targets beta1 integrins: Functional similarity to Yersinia invasin","volume":"286","author":"N\u00e4gele V","year":"2011","unstructured":"V N\u00e4gele, , Neisseria meningitidis adhesin NadA targets beta1 integrins: Functional similarity to Yersinia invasin. 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