{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:06:41Z","timestamp":1790867201079,"version":"4.1.0"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,7,9]]},"abstract":"<jats:p>\n                    Erk5 is a mitogen-activated protein kinase, the biological role of which is largely undefined. Therefore, we deleted the\n                    <jats:italic>erk5<\/jats:italic>\n                    gene in mice to assess its function\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Inactivation of the\n                    <jats:italic>erk5<\/jats:italic>\n                    gene resulted in defective blood-vessel and cardiac development leading to embryonic lethality around embryonic days 9.5\u201310.5. Cardiac development was retarded largely, and the heart failed to undergo normal looping. Endothelial cells that line the developing myocardium of\n                    <jats:italic>erk5<\/jats:italic>\n                    \u2212\/\u2212 embryos displayed a disorganized, rounded morphology. Vasculogenesis occurred, but extraembryonic and embryonic blood vessels were disorganized and failed to mature. Furthermore, the investment of embryonic blood vessels with smooth muscle cells was attenuated. Together, these data define an essential role for Erk5 in cardiovascular development. Moreover, the inability of Erk5-deficient mice to form a complex vasculature suggests that Erk5 may play an important role in controlling angiogenesis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.142293999","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T18:26:28Z","timestamp":1027880788000},"page":"9248-9253","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":205,"title":["Erk5 null mice display multiple extraembryonic vascular and embryonic cardiovascular defects"],"prefix":"10.1073","volume":"99","author":[{"given":"Christopher P.","family":"Regan","sequence":"first","affiliation":[{"name":"Vertex Pharmaceuticals, Department of Biology, 130 Waverly Street, Cambridge, MA 02139"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Vertex Pharmaceuticals, Department of Biology, 130 Waverly Street, Cambridge, MA 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