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The data presented here show that alteration of the ATP binding site of the pseudokinase domain has the potential to suppress JAK hyperactivation caused by pathogenic mutations, with minimal effects on wild-type JAK, thus establishing the ATP binding site of the pseudokinase domain as a potential pharmacological target.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1423201112","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T05:46:36Z","timestamp":1427867196000},"page":"4642-4647","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":111,"title":["ATP binding to the pseudokinase domain of JAK2 is critical for pathogenic activation"],"prefix":"10.1073","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8534-2530","authenticated-orcid":false,"given":"Henrik M.","family":"Hammar\u00e9n","sequence":"first","affiliation":[{"name":"School of Medicine, University of Tampere, FI-33014 Tampere, Finland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Ungureanu","sequence":"additional","affiliation":[{"name":"School of Medicine, University of Tampere, FI-33014 Tampere, Finland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Grisouard","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Experimental Hematology, University Hospital Basel, CH-4031 Basel, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radek C.","family":"Skoda","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Experimental Hematology, University Hospital Basel, CH-4031 Basel, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stevan R.","family":"Hubbard","sequence":"additional","affiliation":[{"name":"Kimmel Center for Biology and Medicine at the Skirball Institute and"},{"name":"Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olli","family":"Silvennoinen","sequence":"additional","affiliation":[{"name":"School of Medicine, University of Tampere, FI-33014 Tampere, Finland;"},{"name":"Clinical Hematology, Department of Internal Medicine, Tampere University Hospital, FI-33520 Tampere, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,3,30]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205156200"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.3.947-956.2000"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.160130297"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2014.07.003"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1401180111"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-06-0342"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.10.3387-3395.2000"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.347"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)71142-9"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03546"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa051113"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.03.023"},{"key":"e_1_3_3_13_2","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1111\/j.1582-4934.2010.01018.x","article-title":"Perspectives for the use of structural information and chemical genetics to develop inhibitors of Janus kinases","volume":"14","author":"Haan C","year":"2010","unstructured":"C Haan, I Behrmann, S Haan, Perspectives for the use of structural information and chemical genetics to develop inhibitors of Janus kinases. 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