{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:04:18Z","timestamp":1790435058995,"version":"4.1.0"},"reference-count":45,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,2,10]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Human cytomegalovirus (HCMV) is a major cause of morbidity and mortality in transplant patients and in fetuses after congenital infection. gH\/gL\/gO and Pentamer are targets for neutralizing antibodies. We show that gO and UL128\/UL130\/UL131A bind to the same site on gH\/gL through formation of a disulfide bond with gL-Cys144. The alternative use of this binding site by either gO or the ULs may provide a mechanism for cell tropism modulation. Our analysis reveals that gH\/gL antigenic sites are conserved among gH\/gL, gH\/gL\/gO, and Pentamer, whereas gH\/gL\/gO- and Pentamer-specific neutralizing antibody-binding sites are located in the gH\/gL N terminus protrusion that contains the gO and the UL subunits. These data support the development of vaccines and antibody therapeutics against HCMV.<\/jats:p>","DOI":"10.1073\/pnas.1424818112","type":"journal-article","created":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T21:56:18Z","timestamp":1422309378000},"page":"1767-1772","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":149,"title":["Structural and biochemical studies of HCMV gH\/gL\/gO and Pentamer reveal mutually exclusive cell entry complexes"],"prefix":"10.1073","volume":"112","author":[{"given":"Claudio","family":"Ciferri","sequence":"first","affiliation":[{"name":"Novartis Vaccines, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumana","family":"Chandramouli","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo","family":"Donnarumma","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel A.","family":"Nikitin","sequence":"additional","affiliation":[{"name":"Novartis Institutes for Biomedical Research, Emeryville, CA 94608;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Cianfrocco","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138; and"},{"name":"Department of Cell Biology, Harvard Medical School, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Gerrein","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam L.","family":"Feire","sequence":"additional","affiliation":[{"name":"Novartis Institutes for Biomedical Research, Emeryville, CA 94608;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan W.","family":"Barnett","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders E.","family":"Lilja","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rino","family":"Rappuoli","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Norais","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, 53100 Siena, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ethan C.","family":"Settembre","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Carfi","sequence":"additional","affiliation":[{"name":"Novartis Vaccines, Cambridge, MA 02139;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,1,26]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/12.Supplement_7.S701"},{"key":"e_1_3_3_2_2","first-page":"417","article-title":"Manifestations of human cytomegalovirus infection: Proposed mechanisms of acute and chronic disease","volume":"325","author":"Britt W","year":"2008","unstructured":"W Britt, Manifestations of human cytomegalovirus infection: Proposed mechanisms of acute and chronic disease. 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