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We investigate how antigen load affects the CD8 response by analyzing the viral load and the magnitude of the specific CD8 response after immunization with the live attenuated yellow fever vaccine (YFV-17D). Our results show that the magnitude of the CD8 response is proportional to the amount of antigen when virus load is below a threshold value and saturates above. This finding highlights the requirement that T cell-based vaccines deliver sufficient antigen to elicit a large CD8 response that may be needed for protection.<\/jats:p>","DOI":"10.1073\/pnas.1500475112","type":"journal-article","created":{"date-parts":[[2015,2,25]],"date-time":"2015-02-25T17:27:01Z","timestamp":1424885221000},"page":"3050-3055","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":134,"title":["Initial viral load determines the magnitude of the human CD8 T cell response to yellow fever vaccination"],"prefix":"10.1073","volume":"112","author":[{"given":"Rama S.","family":"Akondy","sequence":"first","affiliation":[{"name":"Emory Vaccine Center,"},{"name":"Department of Microbiology and Immunology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip L. 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