{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T11:43:00Z","timestamp":1792237380009,"version":"4.4.0"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"15","license":[{"start":{"date-parts":[[2015,3,30]],"date-time":"2015-03-30T00:00:00Z","timestamp":1427673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31225006"],"award-info":[{"award-number":["31225006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,4,14]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>The membrane-anchored GTPase atlastin (ATL) mediates the fusion of endoplasmic reticulum membranes into a network of tubules and sheets, but the mechanism of ATL function is still poorly understood. Here we show that vesicle fusion is preceded by GTP hydrolysis-dependent tethering, caused by the interaction of ATL molecules in opposing membranes. GTP hydrolysis also dissociates ATL dimers sitting in the same membrane (cis dimers), generating a pool of ATL monomers that can dimerize with molecules on a different (trans) membrane. Multiple rounds of GTP hydrolysis and the cooperation of several ATL molecules in each membrane are required for a successful fusion event. These results lead to a model of ATL-mediated fusion that also may have implications for SNARE-mediated fusion.<\/jats:p>","DOI":"10.1073\/pnas.1504368112","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T05:46:36Z","timestamp":1427867196000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":56,"title":["Cis and trans interactions between atlastin molecules during membrane fusion"],"prefix":"10.1073","volume":"112","author":[{"given":"Tina Y.","family":"Liu","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Bian","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Protein Sciences, Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian B.","family":"Romano","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Shemesh","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Technion\u2013Israel Institute of Technology, Haifa 32000, Israel; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom A.","family":"Rapoport","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjie","family":"Hu","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Protein Sciences, Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China;"},{"name":"National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,3,30]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(01)05004-5"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2011.07.004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.11.022"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1456"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1451"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1161748"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11320"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.05.025"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08280"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1313"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3266"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn046"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1101643108"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1012792108"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201308001"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201111115"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.224501"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(08)70258-8"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.353"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-014-0118-0"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201105006"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1105056108"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019194108"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1208385109"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e14-08-1284"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.10.030"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2523"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI40979"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.97"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1407435111"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1237"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.02.029"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1504368112","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1504368112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:27:44Z","timestamp":1649809664000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1504368112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,30]]},"references-count":33,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2015,4,14]]}},"alternative-id":["10.1073\/pnas.1504368112"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1504368112","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,30]]},"assertion":[{"value":"2015-03-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}