{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T01:30:59Z","timestamp":1794360659153,"version":"4.9.0"},"reference-count":44,"publisher":"National Academy of Sciences","issue":"23","license":[{"start":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T00:00:00Z","timestamp":1432598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000138","name":"U.S. Department of Education","doi-asserted-by":"publisher","award":["H133B031118"],"award-info":[{"award-number":["H133B031118"]}],"id":[{"id":"10.13039\/100000138","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000138","name":"U.S. Department of Education","doi-asserted-by":"publisher","award":["H133B090001"],"award-info":[{"award-number":["H133B090001"]}],"id":[{"id":"10.13039\/100000138","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH-09-1-0592"],"award-info":[{"award-number":["W81XWH-09-1-0592"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["UL1RR031988"],"award-info":[{"award-number":["UL1RR031988"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["U54HD053177"],"award-info":[{"award-number":["U54HD053177"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["UL1RR024992"],"award-info":[{"award-number":["UL1RR024992"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["U54RR026139"],"award-info":[{"award-number":["U54RR026139"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["2U54HD053177"],"award-info":[{"award-number":["2U54HD053177"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["G12RR003051"],"award-info":[{"award-number":["G12RR003051"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AR062380"],"award-info":[{"award-number":["R01AR062380"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["P50AR060836"],"award-info":[{"award-number":["P50AR060836"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R24HD050846"],"award-info":[{"award-number":["R24HD050846"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["P30HD040677"],"award-info":[{"award-number":["P30HD040677"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["UL1TR000075"],"award-info":[{"award-number":["UL1TR000075"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,6,9]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Duchenne muscular dystrophy (DMD) is a rare and devastating muscle disease caused by mutations in the X-linked\n                    <jats:italic>DMD<\/jats:italic>\n                    gene (which encodes the dystrophin protein). Serum biomarkers hold significant potential as objective phenotypic measures of DMD disease state, as well as potential measures of pharmacological effects of and response to therapeutic interventions. Here we describe a proteomics approach to determine serum levels of 1,125 proteins in 93 DMD patients and 45 controls. The study identified 44 biomarkers that differed significantly between patients and controls. These data are being made available to DMD researchers and clinicians to accelerate the search for new diagnostic, prognostic, and therapeutic approaches.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1507719112","type":"journal-article","created":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T10:35:29Z","timestamp":1432722929000},"page":"7153-7158","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":279,"title":["Large-scale serum protein biomarker discovery in Duchenne muscular dystrophy"],"prefix":"10.1073","volume":"112","author":[{"given":"Yetrib","family":"Hathout","sequence":"first","affiliation":[{"name":"Research Center for Genetic Medicine, Children\u2019s National Medical Center, Washington, DC 20012;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Brody","sequence":"additional","affiliation":[{"name":"SomaLogic, Inc., Boulder, CO 80301;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula R.","family":"Clemens","sequence":"additional","affiliation":[{"name":"Neurology Service, Department of Veteran Affairs Medical Center, Pittsburgh, PA 15240;"},{"name":"University of Pittsburgh, Pittsburgh, PA 15213;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Cripe","sequence":"additional","affiliation":[{"name":"The Heart Center, Nationwide Children\u2019s Hospital, The Ohio State University, Columbus, OH 15213;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert Kirk","family":"DeLisle","sequence":"additional","affiliation":[{"name":"SomaLogic, Inc., Boulder, CO 80301;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pat","family":"Furlong","sequence":"additional","affiliation":[{"name":"Parent Project Muscular Dystrophy, Hackensack, NJ 07601;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Gordish-Dressman","sequence":"additional","affiliation":[{"name":"Research Center for Genetic Medicine, Children\u2019s National Medical Center, Washington, DC 20012;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Hache","sequence":"additional","affiliation":[{"name":"Research Center for Genetic Medicine, Children\u2019s National Medical Center, Washington, DC 20012;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Henricson","sequence":"additional","affiliation":[{"name":"Department of Physical Medicine and Rehabilitation, University of California Davis School of Medicine, Davis, CA 95618;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric P.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Research Center for Genetic Medicine, Children\u2019s National Medical Center, Washington, DC 20012;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne Monique","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN 46202;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Lorts","sequence":"additional","affiliation":[{"name":"The Heart Institute, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, OH 45229;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean K.","family":"Mah","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Calgary, Alberta Children\u2019s Hospital, Calgary, AB, Canada T3B 6A8;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"McDonald","sequence":"additional","affiliation":[{"name":"Department of Physical Medicine and Rehabilitation, University of California Davis School of Medicine, Davis, CA 95618;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bob","family":"Mehler","sequence":"additional","affiliation":[{"name":"SomaLogic, Inc., Boulder, CO 80301;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally","family":"Nelson","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Denver, Aurora, CO 80045;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malti","family":"Nikrad","sequence":"additional","affiliation":[{"name":"SomaLogic, Inc., Boulder, CO 80301;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britta","family":"Singer","sequence":"additional","affiliation":[{"name":"SomaLogic, Inc., Boulder, CO 80301;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fintan","family":"Steele","sequence":"additional","affiliation":[{"name":"SomaLogic, Inc., Boulder, CO 80301;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sterling","sequence":"additional","affiliation":[{"name":"SomaLogic, Inc., Boulder, CO 80301;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. Lee","family":"Sweeney","sequence":"additional","affiliation":[{"name":"Department of Pharmacology &amp; Therapeutics, University of Florida College of Medicine, Gainesville, FL 32610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Williams","sequence":"additional","affiliation":[{"name":"SomaLogic, Inc., Boulder, CO 80301;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry","family":"Gold","sequence":"additional","affiliation":[{"name":"SomaLogic, Inc., Boulder, CO 80301;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,5,26]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2014.03.006"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(09)70271-6"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2014.03.008"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.122.4.809"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90579-4"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/088307380201700306"},{"key":"e_1_3_3_7_2","first-page":"16","article-title":"The Canadian experience with long-term deflazacort treatment in Duchenne muscular dystrophy","volume":"31","author":"McAdam LC","year":"2012","unstructured":"LC McAdam, AL Mayo, BA Alman, WD Biggar, The Canadian experience with long-term deflazacort treatment in Duchenne muscular dystrophy. Acta Myol 31, 16\u201320 (2012).","journal-title":"Acta Myol"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1002\/mus.23808","article-title":"The cooperative international neuromuscular research group Duchenne natural history study: Glucocorticoid treatment preserves clinically meaningful functional milestones and reduces rate of disease progression as measured by manual muscle testing and other commonly used clinical trial outcome measures","volume":"48","author":"Henricson EK","year":"2013","unstructured":"EK Henricson, , The cooperative international neuromuscular research group Duchenne natural history study: Glucocorticoid treatment preserves clinically meaningful functional milestones and reduces rate of disease progression as measured by manual muscle testing and other commonly used clinical trial outcome measures. Muscle Nerve; CINRG Investigators 48, 55\u201367 (2013).","journal-title":"Muscle Nerve"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmr.2012.08.003"},{"key":"e_1_3_3_10_2","first-page":"CD003725","article-title":"Glucocorticoid corticosteroids for Duchenne muscular dystrophy","author":"Manzur AY","year":"2008","unstructured":"AY Manzur, T Kuntzer, M Pike, A Swan, Glucocorticoid corticosteroids for Duchenne muscular dystrophy. Cochrane Database Syst Rev, pp. CD003725 (2008).","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60756-3"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1011367"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23982"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1000228"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2014.866087"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-120611-144851"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3460"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201302621"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23989"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.23902"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21544"},{"key":"e_1_3_3_22_2","first-page":"233","article-title":"Orphan drug development in muscular dystrophy: Update on two large clinical trials of dystrophin rescue therapies","volume":"16","author":"Hoffman EP","year":"2013","unstructured":"EP Hoffman, EM Connor, Orphan drug development in muscular dystrophy: Update on two large clinical trials of dystrophin rescue therapies. Discov Med 16, 233\u2013239 (2013).","journal-title":"Discov Med"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.126706"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1235"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu366"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201303724"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015004"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbt.2011.11.016"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/mtna.2014.49"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.532697"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1213933109"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.532580"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.23807"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0960-8966(94)00045-B"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar4583"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-7431(03)00098-8"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.04.015"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1309907110"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1097\/SPC.0000000000000013"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-2796com"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009176"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2007.159"},{"key":"e_1_3_3_43_2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: A practical and powerful approach to multiple testing","volume":"57","author":"Benjamini Y","year":"1997","unstructured":"Y Benjamini, Y Hochberg, Controlling the false discovery rate: A practical and powerful approach to multiple testing. J R Stat Soc, B 57, 289\u2013300 (1997).","journal-title":"J R Stat Soc, B"},{"key":"e_1_3_3_44_2","unstructured":"; R Core Team R: A Language and Environment for Statistical Computing . (R Foundation for Statistical Computing Vienna Austria). Available at www.R-project.org\/. Accessed May 7 2015. (2014)."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1507719112","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1507719112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T02:52:42Z","timestamp":1717901562000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1507719112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,26]]},"references-count":44,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2015,6,9]]}},"alternative-id":["10.1073\/pnas.1507719112"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1507719112","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,26]]},"assertion":[{"value":"2015-05-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}