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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,6,9]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    We studied the physiological function of the heterogeneous nuclear ribonucleoprotein U (hnRNP U) by generating a conditional knockout mouse in which the\n                    <jats:italic>Hnrnpu<\/jats:italic>\n                    gene is deleted in the heart. We found that hnRNP U is required for normal pre-mRNA splicing and postnatal heart development and function. Mutant mice develop severe dilated cardiomyopathy and die 2 wk after birth. Phenotypic characterization of mutant hearts coupled with RNA-seq data analyses revealed that mutant hearts display multiple cardiac defects as a result of misregulated gene expression and abnormal pre-mRNA splicing. We also identified the sarcoplasmic reticulum membrane protein Junctin as a splicing target of hnRNP U and provide an interesting example of alternative splicing in controlling the modification and function of proteins.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1508461112","type":"journal-article","created":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T10:35:29Z","timestamp":1432722929000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":116,"title":["hnRNP U protein is required for normal pre-mRNA splicing and postnatal heart development and function"],"prefix":"10.1073","volume":"112","author":[{"given":"Junqiang","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Beetz","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390;"},{"name":"Institute of Experimental and Clinical Pharmacology and Toxicology, University of Freiburg, 79104 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"O\u2019Keeffe","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Tapia","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsey","family":"Macpherson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weisheng V.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhonda","family":"Bassel-Duby","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric N.","family":"Olson","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Maniatis","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,5,26]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1146\/annurev.biochem.72.121801.161720","article-title":"Mechanisms of alternative pre-messenger RNA splicing","volume":"72","author":"Black DL","year":"2003","unstructured":"DL Black, Mechanisms of alternative pre-messenger RNA splicing. 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