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The development of chemoenzymatic or semisynthetic means of manufacture requires elucidation of at least four central, undefined steps in the biosynthesis of thienamycin. The cobalamin-dependent radical\n                    <jats:italic>S<\/jats:italic>\n                    -adenosylmethionine (RS) superfamily enzyme ThnK performs two sequential methyl transfers in a stereodefined manner. This enzyme activity identifies critical intermediates in carbapenem biosynthesis, more clearly defining the enigmatic central steps of the pathway and exemplifying an unprecedented reaction sequence among RS enzymes.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1508615112","type":"journal-article","created":{"date-parts":[[2015,8,3]],"date-time":"2015-08-03T22:49:47Z","timestamp":1438642187000},"page":"10354-10358","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":93,"title":["Consecutive radical\n                    <i>S<\/i>\n                    -adenosylmethionine methylations form the ethyl side chain in thienamycin biosynthesis"],"prefix":"10.1073","volume":"112","author":[{"given":"Daniel R.","family":"Marous","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan P.","family":"Lloyd","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R.","family":"Buller","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristos A.","family":"Moshos","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyler L.","family":"Grove","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The Pennsylvania State University, University Park, PA 16802;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony J.","family":"Blaszczyk","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Squire J.","family":"Booker","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The Pennsylvania State University, University Park, PA 16802;"},{"name":"Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig A.","family":"Townsend","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,8,3]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1128"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201100366"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja001723l"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804500105"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ar200202c"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr4004709"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/10409230701829169"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/07388558609150792"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1091"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja312641f"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2013.12.004"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2014.12.012"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.35.1255"},{"key":"e_1_3_3_14_2","first-page":"1","article-title":"Spectroscopic characterization and mechanistic investigation of P-methyl transfer by a radical SAM enzyme from the marine bacterium Shewanella denitrificans OS217","volume":"1844","author":"Allen KD","year":"2014","unstructured":"KD Allen, SC Wang, Spectroscopic characterization and mechanistic investigation of P-methyl transfer by a radical SAM enzyme from the marine bacterium Shewanella denitrificans OS217. 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