{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:03:18Z","timestamp":1791295398136,"version":"4.1.0"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"25","license":[{"start":{"date-parts":[[2015,6,8]],"date-time":"2015-06-08T00:00:00Z","timestamp":1433721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/501100000781","name":"EC | European Research Council","doi-asserted-by":"publisher","award":["232798"],"award-info":[{"award-number":["232798"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"EC | European Research Council","doi-asserted-by":"publisher","award":["339579"],"award-info":[{"award-number":["339579"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","award":["senior foreign scholarship"],"award-info":[{"award-number":["senior foreign scholarship"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002271","name":"Pasteur Foundation","doi-asserted-by":"publisher","award":["post-doctoral fellowship"],"award-info":[{"award-number":["post-doctoral fellowship"]}],"id":[{"id":"10.13039\/100002271","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003043","name":"EMBO","doi-asserted-by":"publisher","award":["post-doctoral fellowship"],"award-info":[{"award-number":["post-doctoral fellowship"]}],"id":[{"id":"10.13039\/501100003043","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,6,23]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Shigella<\/jats:italic>\n                    spp\n                    <jats:italic>.<\/jats:italic>\n                    are responsible for devastating diarrheal diseases, primarily in children, within underdeveloped countries.\n                    <jats:italic>Shigella<\/jats:italic>\n                    invades the mucosa of the large intestine, causing inflammation and damage to the epithelium. Here, we have measured the progression of\n                    <jats:italic>Shigella<\/jats:italic>\n                    infection in a small animal model of the disease to better understand the mechanism of invasion of the colonic mucosa. The novelty of our approach relies on the tracking of fluorescent bacteria inside the infected tissue at various time points using confocal microscopy and subsequent quantitative bioimage analyses. Our approach is readily applicable to other host\u2013pathogen systems to quantify host\u2013pathogen interactions.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1509091112","type":"journal-article","created":{"date-parts":[[2015,6,10]],"date-time":"2015-06-10T13:23:59Z","timestamp":1433942639000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":74,"title":["Bioimage analysis of\n                    <i>Shigella<\/i>\n                    infection reveals targeting of colonic crypts"],"prefix":"10.1073","volume":"112","author":[{"given":"Ellen T.","family":"Arena","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM, U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois-Xavier","family":"Campbell-Valois","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM, U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Yves","family":"Tinevez","sequence":"additional","affiliation":[{"name":"Center for Innovation &amp; Technological Research, Imagopole\u2013Plateforme D\u2019Imagerie Dynamique, Institut Pasteur, 75724 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Nigro","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM, U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Sachse","sequence":"additional","affiliation":[{"name":"Center for Innovation &amp; Technological Research, Ultrapole, Institut Pasteur, 75724 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryse","family":"Moya-Nilges","sequence":"additional","affiliation":[{"name":"Center for Innovation &amp; Technological Research, Ultrapole, Institut Pasteur, 75724 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Nothelfer","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM, U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Marteyn","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM, U1202, 75015 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spencer L.","family":"Shorte","sequence":"additional","affiliation":[{"name":"Center for Innovation &amp; Technological Research, Imagopole\u2013Plateforme D\u2019Imagerie Dynamique, Institut Pasteur, 75724 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe J.","family":"Sansonetti","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur, 75724 Paris, France;"},{"name":"INSERM, U1202, 75015 Paris, France;"},{"name":"Coll\u00e8ge de France, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,6,8]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5247.315"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12032"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.12.005"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2014.05.022"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.10.009"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/13.Supplement_4.S314"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2008.12.002"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090124"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3867"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.01.005"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.4.2476"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1258\/002367781780958577"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1896"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130914"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.365"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(91)90128-S"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_17010123.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.10.5240"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.03.001"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1423"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504466102"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/smim.1999.0175"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01478-2"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(99)00051-X"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.123"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.042"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.35.3.852-860.1982"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01097.x"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01770.x"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036446"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004713"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-416026-2.00004-2"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1509091112","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1509091112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:03:50Z","timestamp":1649808230000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1509091112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,8]]},"references-count":33,"journal-issue":{"issue":"25","published-print":{"date-parts":[[2015,6,23]]}},"alternative-id":["10.1073\/pnas.1509091112"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1509091112","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,8]]},"assertion":[{"value":"2015-06-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}