{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:41:24Z","timestamp":1790836884235,"version":"4.1.0"},"reference-count":39,"publisher":"National Academy of Sciences","issue":"33","license":[{"start":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T00:00:00Z","timestamp":1454457600000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI055058"],"award-info":[{"award-number":["AI055058"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI058935"],"award-info":[{"award-number":["AI058935"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,8,18]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    Bacterial pathogens evolve rapidly in the face of clinical interventions and therapeutics; one mechanism that can promote this evolution is their ability to acquire novel DNA sequences, known as horizontal gene transfer (HGT). Here, we studied HGT in clinical isolates of\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    , the causative agent of cholera, and found that a horizontally transferred element inhibits another mechanism of HGT\u2014natural transformation. The element that inhibits natural transformation is globally distributed among\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    isolates. We show, however, that there has been a rise in the prevalence of strains that lack this inhibitory element. Thus, our results suggest that in the future there may be an increase in the role of natural transformation on the evolution of this pathogen.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1509097112","type":"journal-article","created":{"date-parts":[[2015,8,3]],"date-time":"2015-08-03T22:49:47Z","timestamp":1438642187000},"page":"10485-10490","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":65,"title":["A globally distributed mobile genetic element inhibits natural transformation of\n                    <i>Vibrio cholerae<\/i>"],"prefix":"10.1073","volume":"112","author":[{"given":"Ankur B.","family":"Dalia","sequence":"first","affiliation":[{"name":"Department of Molecular Biology &amp; Microbiology, Tufts University School of Medicine, Boston, MA 02111;"},{"name":"Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, MA 02111;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberley D.","family":"Seed","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, &amp; Developmental Biology, University of Michigan, Ann Arbor, MI 48109;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen B.","family":"Calderwood","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Camilli","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology &amp; Microbiology, Tufts University School of Medicine, Boston, MA 02111;"},{"name":"Howard Hughes Medical Institute, Tufts University School of Medicine, Boston, MA 02111;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,8,3]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"2466","DOI":"10.1016\/S0140-6736(12)60436-X","article-title":"Cholera","volume":"379","author":"Harris JB","year":"2012","unstructured":"JB Harris, RC LaRocque, F Qadri, ET Ryan, SB Calderwood, Cholera. Lancet 379, 2466\u20132476 (2012).","journal-title":"Lancet"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1111\/j.1462-2920.2007.01559.x","article-title":"Global impact of Vibrio cholerae interactions with chitin","volume":"10","author":"Pruzzo C","year":"2008","unstructured":"C Pruzzo, L Vezzulli, RR Colwell, Global impact of Vibrio cholerae interactions with chitin. Environ Microbiol 10, 1400\u20131410 (2008).","journal-title":"Environ Microbiol"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1128\/aem.45.1.275-283.1983","article-title":"Ecological relationships between Vibrio cholerae and planktonic crustacean copepods","volume":"45","author":"Huq A","year":"1983","unstructured":"A Huq, , Ecological relationships between Vibrio cholerae and planktonic crustacean copepods. Appl Environ Microbiol 45, 275\u2013283 (1983).","journal-title":"Appl Environ Microbiol"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1126\/science.274.5295.2025","article-title":"Global climate and infectious disease: The cholera paradigm","volume":"274","author":"Colwell RR","year":"1996","unstructured":"RR Colwell, Global climate and infectious disease: The cholera paradigm. Science 274, 2025\u20132031 (1996).","journal-title":"Science"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1126\/science.1120096","article-title":"Chitin induces natural competence in Vibrio cholerae","volume":"310","author":"Meibom KL","year":"2005","unstructured":"KL Meibom, M Blokesch, NA Dolganov, CY Wu, GK Schoolnik, Chitin induces natural competence in Vibrio cholerae. Science 310, 1824\u20131827 (2005).","journal-title":"Science"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"e81","DOI":"10.1371\/journal.ppat.0030081","article-title":"Serogroup conversion of Vibrio cholerae in aquatic reservoirs","volume":"3","author":"Blokesch M","year":"2007","unstructured":"M Blokesch, GK Schoolnik, Serogroup conversion of Vibrio cholerae in aquatic reservoirs. PLoS Pathog 3, e81 (2007).","journal-title":"PLoS Pathog"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"10374","DOI":"10.1073\/pnas.92.22.10374","article-title":"Genetic rearrangements in the rfb regions of Vibrio cholerae O1 and O139","volume":"92","author":"Stroeher UH","year":"1995","unstructured":"UH Stroeher, , Genetic rearrangements in the rfb regions of Vibrio cholerae O1 and O139. Proc Natl Acad Sci USA 92, 10374\u201310378 (1995).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_8_2","doi-asserted-by":"crossref","first-page":"11388","DOI":"10.1073\/pnas.91.24.11388","article-title":"The Vibrio cholerae O139 serogroup antigen includes an O-antigen capsule and lipopolysaccharide virulence determinants","volume":"91","author":"Waldor MK","year":"1994","unstructured":"MK Waldor, R Colwell, JJ Mekalanos, The Vibrio cholerae O139 serogroup antigen includes an O-antigen capsule and lipopolysaccharide virulence determinants. Proc Natl Acad Sci USA 91, 11388\u201311392 (1994).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_9_2","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1016\/S0140-6736(94)92504-6","article-title":"Vibrio cholerae O139 specific gene sequences","volume":"343","author":"Waldor MK","year":"1994","unstructured":"MK Waldor, JJ Mekalanos, Vibrio cholerae O139 specific gene sequences. Lancet 343, 1366 (1994).","journal-title":"Lancet"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"01356","DOI":"10.1128\/mBio.01356-14","article-title":"Acquisition and evolution of SXT-R391 integrative conjugative elements in the seventh-pandemic Vibrio cholerae lineage","volume":"5","author":"Spagnoletti M","year":"2014","unstructured":"M Spagnoletti, , Acquisition and evolution of SXT-R391 integrative conjugative elements in the seventh-pandemic Vibrio cholerae lineage. MBio 5, 01356\u201314 (2014).","journal-title":"MBio"},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"e1000786","DOI":"10.1371\/journal.pgen.1000786","article-title":"Comparative ICE genomics: Insights into the evolution of the SXT\/R391 family of ICEs","volume":"5","author":"Wozniak RA","year":"2009","unstructured":"RA Wozniak, , Comparative ICE genomics: Insights into the evolution of the SXT\/R391 family of ICEs. PLoS Genet 5, e1000786 (2009).","journal-title":"PLoS Genet"},{"key":"e_1_3_4_12_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1046\/j.1365-2958.1999.01330.x","article-title":"Site-specific integration of the conjugal Vibrio cholerae SXT element into prfC","volume":"32","author":"Hochhut B","year":"1999","unstructured":"B Hochhut, MK Waldor, Site-specific integration of the conjugal Vibrio cholerae SXT element into prfC. Mol Microbiol 32, 99\u2013110 (1999).","journal-title":"Mol Microbiol"},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1038\/nature10392","article-title":"Evidence for several waves of global transmission in the seventh cholera pandemic","volume":"477","author":"Mutreja A","year":"2011","unstructured":"A Mutreja, , Evidence for several waves of global transmission in the seventh cholera pandemic. Nature 477, 462\u2013465 (2011).","journal-title":"Nature"},{"key":"e_1_3_4_14_2","doi-asserted-by":"crossref","first-page":"e1005072","DOI":"10.1371\/journal.pgen.1005072","article-title":"The role of China in the global spread of the current cholera pandemic","volume":"11","author":"Didelot X","year":"2015","unstructured":"X Didelot, , The role of China in the global spread of the current cholera pandemic. PLoS Genet 11, e1005072 (2015).","journal-title":"PLoS Genet"},{"key":"e_1_3_4_15_2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1056\/NEJMoa1012928","article-title":"The origin of the Haitian cholera outbreak strain","volume":"364","author":"Chin CS","year":"2011","unstructured":"CS Chin, , The origin of the Haitian cholera outbreak strain. N Engl J Med 364, 33\u201342 (2011).","journal-title":"N Engl J Med"},{"key":"e_1_3_4_16_2","first-page":"00398","article-title":"Evolutionary dynamics of Vibrio cholerae O1 following a single-source introduction to Haiti","volume":"4","author":"Katz LS","year":"2013","unstructured":"LS Katz, , Evolutionary dynamics of Vibrio cholerae O1 following a single-source introduction to Haiti. MBio 4, 00398\u201313 (2013).","journal-title":"MBio"},{"key":"e_1_3_4_17_2","doi-asserted-by":"crossref","first-page":"01824","DOI":"10.1128\/mBio.01824-14","article-title":"Phylodynamic analysis of clinical and environmental Vibrio cholerae isolates from Haiti reveals diversification driven by positive selection","volume":"5","author":"Azarian T","year":"2014","unstructured":"T Azarian, , Phylodynamic analysis of clinical and environmental Vibrio cholerae isolates from Haiti reveals diversification driven by positive selection. MBio 5, 01824\u201314 (2014).","journal-title":"MBio"},{"key":"e_1_3_4_18_2","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1128\/jb.171.3.1288-1293.1989","article-title":"Identification of toxS, a regulatory gene whose product enhances toxR-mediated activation of the cholera toxin promoter","volume":"171","author":"Miller VL","year":"1989","unstructured":"VL Miller, VJ DiRita, JJ Mekalanos, Identification of toxS, a regulatory gene whose product enhances toxR-mediated activation of the cholera toxin promoter. J Bacteriol 171, 1288\u20131293 (1989).","journal-title":"J Bacteriol"},{"key":"e_1_3_4_19_2","doi-asserted-by":"crossref","first-page":"7232","DOI":"10.1128\/JB.00959-08","article-title":"The extracellular nuclease Dns and its role in natural transformation of Vibrio cholerae","volume":"190","author":"Blokesch M","year":"2008","unstructured":"M Blokesch, GK Schoolnik, The extracellular nuclease Dns and its role in natural transformation of Vibrio cholerae. J Bacteriol 190, 7232\u20137240 (2008).","journal-title":"J Bacteriol"},{"key":"e_1_3_4_20_2","doi-asserted-by":"crossref","first-page":"e1004043","DOI":"10.1371\/journal.ppat.1004043","article-title":"Concerted spatio-temporal dynamics of imported DNA and ComE DNA uptake protein during gonococcal transformation","volume":"10","author":"Gangel H","year":"2014","unstructured":"H Gangel, , Concerted spatio-temporal dynamics of imported DNA and ComE DNA uptake protein during gonococcal transformation. PLoS Pathog 10, e1004043 (2014).","journal-title":"PLoS Pathog"},{"key":"e_1_3_4_21_2","doi-asserted-by":"crossref","first-page":"17987","DOI":"10.1073\/pnas.1315647110","article-title":"DNA-uptake machinery of naturally competent Vibrio cholerae","volume":"110","author":"Seitz P","year":"2013","unstructured":"P Seitz, M Blokesch, DNA-uptake machinery of naturally competent Vibrio cholerae. Proc Natl Acad Sci USA 110, 17987\u201317992 (2013).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_22_2","doi-asserted-by":"crossref","first-page":"e1004066","DOI":"10.1371\/journal.pgen.1004066","article-title":"ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholerae cells","volume":"10","author":"Seitz P","year":"2014","unstructured":"P Seitz, , ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholerae cells. PLoS Genet 10, e1004066 (2014).","journal-title":"PLoS Genet"},{"key":"e_1_3_4_23_2","doi-asserted-by":"crossref","first-page":"01409","DOI":"10.1128\/mBio.01409-14","article-title":"DNA transport across the outer and inner membranes of naturally transformable Vibrio cholerae is spatially but not temporally coupled","volume":"5","author":"Seitz P","year":"2014","unstructured":"P Seitz, M Blokesch, DNA transport across the outer and inner membranes of naturally transformable Vibrio cholerae is spatially but not temporally coupled. MBio 5, 01409\u201301414 (2014).","journal-title":"MBio"},{"key":"e_1_3_4_24_2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.2144\/000113981","article-title":"Homopolymer tail-mediated ligation PCR: A streamlined and highly efficient method for DNA cloning and library construction","volume":"54","author":"Lazinski DW","year":"2013","unstructured":"DW Lazinski, A Camilli, Homopolymer tail-mediated ligation PCR: A streamlined and highly efficient method for DNA cloning and library construction. Biotechniques 54, 25\u201334 (2013).","journal-title":"Biotechniques"},{"key":"e_1_3_4_25_2","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1038\/35020000","article-title":"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae","volume":"406","author":"Heidelberg JF","year":"2000","unstructured":"JF Heidelberg, , DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature 406, 477\u2013483 (2000).","journal-title":"Nature"},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1046\/j.1365-2958.2003.03702.x","article-title":"Transformation of Streptococcus pneumoniae relies on DprA- and RecA-dependent protection of incoming DNA single strands","volume":"50","author":"Berg\u00e9 M","year":"2003","unstructured":"M Berg\u00e9, I Mortier-Barri\u00e8re, B Martin, JP Claverys, Transformation of Streptococcus pneumoniae relies on DprA- and RecA-dependent protection of incoming DNA single strands. Mol Microbiol 50, 527\u2013536 (2003).","journal-title":"Mol Microbiol"},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1128\/jb.177.11.3235-3240.1995","article-title":"DNA sequence and characterization of Haemophilus influenzae dprA+, a gene required for chromosomal but not plasmid DNA transformation","volume":"177","author":"Karudapuram S","year":"1995","unstructured":"S Karudapuram, X Zhao, GJ Barcak, DNA sequence and characterization of Haemophilus influenzae dprA+, a gene required for chromosomal but not plasmid DNA transformation. J Bacteriol 177, 3235\u20133240 (1995).","journal-title":"J Bacteriol"},{"key":"e_1_3_4_28_2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1038\/nrmicro844","article-title":"DNA uptake during bacterial transformation","volume":"2","author":"Chen I","year":"2004","unstructured":"I Chen, D Dubnau, DNA uptake during bacterial transformation. Nat Rev Microbiol 2, 241\u2013249 (2004).","journal-title":"Nat Rev Microbiol"},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","first-page":"3524","DOI":"10.1128\/JB.00040-10","article-title":"Genome sequence of hybrid Vibrio cholerae O1 MJ-1236, B-33, and CIRS101 and comparative genomics with V. cholerae","volume":"192","author":"Grim CJ","year":"2010","unstructured":"CJ Grim, , Genome sequence of hybrid Vibrio cholerae O1 MJ-1236, B-33, and CIRS101 and comparative genomics with V. cholerae. J Bacteriol 192, 3524\u20133533 (2010).","journal-title":"J Bacteriol"},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"2636","DOI":"10.1128\/JB.186.9.2636-2645.2004","article-title":"Formation of SXT tandem arrays and SXT-R391 hybrids","volume":"186","author":"Burrus V","year":"2004","unstructured":"V Burrus, MK Waldor, Formation of SXT tandem arrays and SXT-R391 hybrids. J Bacteriol 186, 2636\u20132645 (2004).","journal-title":"J Bacteriol"},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"e1000775","DOI":"10.1371\/journal.pgen.1000775","article-title":"Mobile antibiotic resistance encoding elements promote their own diversity","volume":"5","author":"Garriss G","year":"2009","unstructured":"G Garriss, MK Waldor, V Burrus, Mobile antibiotic resistance encoding elements promote their own diversity. PLoS Genet 5, e1000775 (2009).","journal-title":"PLoS Genet"},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"5090","DOI":"10.1128\/IAI.00793-09","article-title":"Memory T-cell responses to Vibrio cholerae O1 infection","volume":"77","author":"Weil AA","year":"2009","unstructured":"AA Weil, , Memory T-cell responses to Vibrio cholerae O1 infection. Infect Immun 77, 5090\u20135096 (2009).","journal-title":"Infect Immun"},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"8937","DOI":"10.1073\/pnas.1406478111","article-title":"Multiplex genome editing by natural transformation","volume":"111","author":"Dalia AB","year":"2014","unstructured":"AB Dalia, E McDonough, A Camilli, Multiplex genome editing by natural transformation. Proc Natl Acad Sci USA 111, 8937\u20138942 (2014).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_34_2","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1038\/nature02241","article-title":"SOS response promotes horizontal dissemination of antibiotic resistance genes","volume":"427","author":"Beaber JW","year":"2004","unstructured":"JW Beaber, B Hochhut, MK Waldor, SOS response promotes horizontal dissemination of antibiotic resistance genes. Nature 427, 72\u201374 (2004).","journal-title":"Nature"},{"key":"e_1_3_4_35_2","doi-asserted-by":"crossref","first-page":"e01028","DOI":"10.1128\/mBio.01028-13","article-title":"Identification of a membrane-bound transcriptional regulator that links chitin and natural competence in Vibrio cholerae","volume":"5","author":"Dalia AB","year":"2014","unstructured":"AB Dalia, DW Lazinski, A Camilli, Identification of a membrane-bound transcriptional regulator that links chitin and natural competence in Vibrio cholerae. MBio 5, e01028\u2013e13 (2014).","journal-title":"MBio"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1007\/BF00424873","article-title":"Differential release of periplasmic versus cytoplasmic enzymes from Escherichia coli B by polymixin B","volume":"78","author":"Cerny G","year":"1971","unstructured":"G Cerny, M Teuber, Differential release of periplasmic versus cytoplasmic enzymes from Escherichia coli B by polymixin B. Arch Mikrobiol 78, 166\u2013179 (1971).","journal-title":"Arch Mikrobiol"},{"key":"e_1_3_4_37_2","doi-asserted-by":"crossref","first-page":"e03497","DOI":"10.7554\/eLife.03497","article-title":"Evolutionary consequences of intra-patient phage predation on microbial populations","volume":"3","author":"Seed KD","year":"2014","unstructured":"KD Seed, , Evolutionary consequences of intra-patient phage predation on microbial populations. eLife 3, e03497 (2014).","journal-title":"eLife"},{"key":"e_1_3_4_38_2","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1093\/molbev\/msu088","article-title":"Automated reconstruction of whole-genome phylogenies from short-sequence reads","volume":"31","author":"Bertels F","year":"2014","unstructured":"F Bertels, OK Silander, M Pachkov, PB Rainey, E van Nimwegen, Automated reconstruction of whole-genome phylogenies from short-sequence reads. Mol Biol Evol 31, 1077\u20131088 (2014).","journal-title":"Mol Biol Evol"},{"key":"e_1_3_4_39_2","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/0378-1119(86)90358-6","article-title":"Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector","volume":"48","author":"F\u00fcrste JP","year":"1986","unstructured":"JP F\u00fcrste, , Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48, 119\u2013131 (1986).","journal-title":"Gene"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1509097112","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1509097112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T09:15:33Z","timestamp":1654593333000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1509097112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,3]]},"references-count":39,"journal-issue":{"issue":"33","published-print":{"date-parts":[[2015,8,18]]}},"alternative-id":["10.1073\/pnas.1509097112"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1509097112","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,3]]},"assertion":[{"value":"2015-08-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}