{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:37:01Z","timestamp":1789166221852,"version":"build-2803163510"},"reference-count":31,"publisher":"National Academy of Sciences","issue":"38","license":[{"start":{"date-parts":[[2016,3,8]],"date-time":"2016-03-08T00:00:00Z","timestamp":1457395200000},"content-version":"vor","delay-in-days":182,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["GM081685"],"award-info":[{"award-number":["GM081685"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,9,22]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Protein palmitoylation has been proposed to mediate the recruitment of signaling proteins into lipid rafts. However, a significant shortcoming of this hypothesis is that the proposed kinetics of protein palmitoylation\/depalmitoylation heretofore have been too slow to mediate very rapid signaling events. Using a combination of a highly sensitive palmitoylation assay with pulsed metabolic labeling, we were able to detect a population of the Src kinase Lck undergoing extremely rapid palmitoylation and depalmitoylation subsequent to Fas receptor stimulation. The rapid rise and fall in Lck palmitoylation was highly synchronized with other signaling events occurring during the first minutes after activation of the Fas pathway, suggesting the existence of a previously undescribed signal transduction mechanism.<\/jats:p>","DOI":"10.1073\/pnas.1509929112","type":"journal-article","created":{"date-parts":[[2015,9,8]],"date-time":"2015-09-08T22:21:21Z","timestamp":1441750881000},"page":"11876-11880","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":87,"title":["Rapid and transient palmitoylation of the tyrosine kinase Lck mediates Fas signaling"],"prefix":"10.1073","volume":"112","author":[{"given":"Askar M.","family":"Akimzhanov","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren","family":"Boehning","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston, Houston, TX 77030"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,9,8]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2084"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/09687680802680108"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-387664-5.00001-7"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/09687680802683839"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2006.03.010"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.13.7705"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(99)00012-8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/09687680802629329"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(99)00007-4"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015045"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/09687680802650481"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803921"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.1.261"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.10.10.5197-5206.1990"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)52927-6"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2478(01)00174-2"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.16.4983"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.6.3226"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.12.6914"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1005419107"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200608035"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/stke.3592006re14"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02311-2"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1293"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.10.3901.3901_3901_3908"},{"key":"e_1_3_3_26_2","doi-asserted-by":"crossref","unstructured":"FJ Dekker Small-molecule inhibition of APT1 affects Ras localization and signaling. Nat Chem Biol 6(6):449\u2013456. (2010).","DOI":"10.1038\/nchembio.362"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01144-08"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107005200"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1063"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409650102"},{"key":"e_1_3_3_31_2","first-page":"135","article-title":"Large-scale profiling of protein palmitoylation in mammalian cells","volume":"6","author":"Martin BR","year":"2009","unstructured":"BR Martin, BF Cravatt, Large-scale profiling of protein palmitoylation in mammalian cells. Mat Methods 6, 135\u2013138 (2009).","journal-title":"Mat Methods"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1509929112","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1509929112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:15:44Z","timestamp":1649808944000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1509929112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,8]]},"references-count":31,"journal-issue":{"issue":"38","published-print":{"date-parts":[[2015,9,22]]}},"alternative-id":["10.1073\/pnas.1509929112"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1509929112","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,8]]},"assertion":[{"value":"2015-09-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}