{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:27:12Z","timestamp":1790432832424,"version":"4.1.0"},"reference-count":38,"publisher":"National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,8,14]]},"abstract":"<jats:p>\n                    Muscarinic acetylcholine receptors exert slow and prolonged\n synaptic effects in both vertebrate and invertebrate nervous systems.\n Through activation of G proteins, they typically decrease intracellular\n cAMP levels by inhibition of adenylate cyclase or stimulate\n phospholipase C and the turnover of inositol phosphates. In insects,\n muscarinic receptors have been credited with two main functions:\n inhibition of transmitter release from sensory neuron terminals and\n regulation of the excitability of motoneurons and interneurons. Our\n pharmacological studies with intact and behaving grasshoppers revealed\n a functional role for muscarinic acetylcholine receptors as being the\n basis for specific arousal in defined areas of the brain, underlying\n the selection and control of acoustic communication behavior. Periodic\n injections of acetylcholine into distinct areas of the brain elicited\n songs of progressively increasing duration. Coinjections of the\n muscarinic receptor antagonist scopolamine and periodic stimulations\n with muscarine identified muscarinic receptor activation as being the\n basis for the underlying accumulation of excitation. In contrast to\n reports from other studies on functional circuits, muscarinic\n excitation was apparently mediated by activation of the adenylate\n cyclase pathway. Stimulation of adenylate cyclase with forskolin and of\n protein kinase A with 8-Br-cAMP mimicked the stimulatory effects of\n muscarine whereas inhibition of adenylate cyclase with SQ22536 and of\n protein kinase A with H-89 and Rp-cAMPs suppressed muscarine-stimulated\n singing behavior. Activation of adenylate cyclase by muscarinic\n receptors has previously been reported from studies on membrane\n preparations and heterologous expression systems, but a physiological\n significance of this pathway remained to be demonstrated in an\n                    <jats:italic>in vivo<\/jats:italic>\n                    preparation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.151131998","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:34:10Z","timestamp":1027679650000},"page":"9919-9923","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":31,"title":["A role for muscarinic excitation: Control of specific singing behavior by activation of the adenylate cyclase pathway in the brain of grasshoppers"],"prefix":"10.1073","volume":"98","author":[{"given":"Ralf","family":"Heinrich","sequence":"first","affiliation":[{"name":"Department of Neurobiology, Institute for Zoology and 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