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Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,8,11]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    (\n                    <jats:italic>Mtb<\/jats:italic>\n                    ) is the most deadly bacterial pathogen in the world, killing 1.5 million people in 2013. Very little is known about the way this pathogen interacts metabolically with its host to achieve long-term persistence and antibiotic tolerance. We uncovered a previously unknown metabolic vulnerability of\n                    <jats:italic>Mtb<\/jats:italic>\n                    , the absolute requirement for methionine and\n                    <jats:italic>S<\/jats:italic>\n                    -adenosylmethionine for successful host infection and virulence. Inactivation of methionine biosynthesis in\n                    <jats:italic>Mtb<\/jats:italic>\n                    leads to unusually rapid cell death, a highly desired feature for chemotherapy. Bactericidal auxotrophies are rare in\n                    <jats:italic>Mtb<\/jats:italic>\n                    , and so far their killing mechanisms have not been characterized systematically. Our study unravels a killing mechanism induced by amino acid starvation that leads to multitarget inhibition, opening new avenues for antimycobacterial interventions.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1513033112","type":"journal-article","created":{"date-parts":[[2015,7,28]],"date-time":"2015-07-28T22:29:49Z","timestamp":1438122589000},"page":"10008-10013","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":151,"title":["Essential roles of methionine and\n                    <i>S<\/i>\n                    -adenosylmethionine in the autarkic lifestyle of\n                    <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1073","volume":"112","author":[{"given":"Michael","family":"Berney","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Berney-Meyer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ka-Wing","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461;"},{"name":"Shanghai Public Health Clinical Center, Key Laboratory of Medical Molecular Virology at Shanghai Medical College, Fudan University, Shanghai 201508, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Chen","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, NY 10461;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Scripps Research Institute, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Harris","sequence":"additional","affiliation":[{"name":"Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"Parkhill","sequence":"additional","affiliation":[{"name":"Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"California Institute for Biomedical Research, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"William R.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461;"},{"name":"Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, NY 10461;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,7,28]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.copbio.2014.10.001","article-title":"Biological insights through nontargeted metabolomics","volume":"34","author":"S\u00e9vin DC","year":"2014","unstructured":"DC S\u00e9vin, A Kuehne, N Zamboni, U Sauer, Biological insights through nontargeted metabolomics. 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