{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:50:44Z","timestamp":1791323444920,"version":"4.3.1"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"1","license":[{"start":{"date-parts":[[2016,6,22]],"date-time":"2016-06-22T00:00:00Z","timestamp":1466553600000},"content-version":"vor","delay-in-days":183,"URL":"http:\/\/www.pnas.org\/preview_site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-12-BSVB-0013-01"],"award-info":[{"award-number":["ANR-12-BSVB-0013-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2016,1,5]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>\n                    The normally biologically unusual CO and CN\n                    <jats:sup>\u2212<\/jats:sup>\n                    diatomic compounds are characteristic iron ligands of hydrogen-metabolizing enzymes. In [FeFe]-hydrogenase, these ligands are synthesized from tyrosine-derived dehydroglycine (DHG) by the radical\n                    <jats:italic>S<\/jats:italic>\n                    -adenosyl-L-methionine (SAM) enzyme HydG. Although the SAM-based cleavage of tyrosine is fairly well understood, the mechanism of CO and CN\n                    <jats:sup>\u2013<\/jats:sup>\n                    synthesis from DHG, which occurs at an unprecedented second iron-sulfur cluster, remains controversial. We report experiments showing that CN\n                    <jats:sup>\u2013<\/jats:sup>\n                    and CO syntheses are independent catalytic events. Whereas CN\n                    <jats:sup>\u2013<\/jats:sup>\n                    synthesis is iron-sulfur cluster-independent, the concomitantly produced\n                    <jats:sup>\u2013<\/jats:sup>\n                    :CO\n                    <jats:sub>2<\/jats:sub>\n                    (H) is converted to CO and H\n                    <jats:sub>2<\/jats:sub>\n                    O at the second cluster. We unambiguously establish this reaction sequence by detecting\n                    <jats:sup>\u2013<\/jats:sup>\n                    :CO\n                    <jats:sub>2<\/jats:sub>\n                    (H)-derived formate that accumulates when the second iron-sulfur cluster is either absent or impaired.\n                  <\/jats:p>","DOI":"10.1073\/pnas.1515842113","type":"journal-article","created":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T20:07:30Z","timestamp":1450901250000},"page":"104-109","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":56,"title":["CO and CN\n                    <sup>\u2212<\/sup>\n                    syntheses by [FeFe]-hydrogenase maturase HydG are catalytically differentiated events"],"prefix":"10.1073","volume":"113","author":[{"given":"Adrien","family":"Pagnier","sequence":"first","affiliation":[{"name":"Metalloproteins Unit, Institut de Biologie Structurale Commissariat \u00e0 l'Energie Atomique et aux Energies Renouvelables\u2013Centre National de la Recherche Scientifique\u2013Universit\u00e9 Grenoble Alpes, 38044 Grenoble Cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydie","family":"Martin","sequence":"additional","affiliation":[{"name":"Metalloproteins Unit, Institut de Biologie Structurale Commissariat \u00e0 l'Energie Atomique et aux Energies Renouvelables\u2013Centre National de la Recherche Scientifique\u2013Universit\u00e9 Grenoble Alpes, 38044 Grenoble Cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Zeppieri","sequence":"additional","affiliation":[{"name":"Metalloproteins Unit, Institut de Biologie Structurale Commissariat \u00e0 l'Energie Atomique et aux Energies Renouvelables\u2013Centre National de la Recherche Scientifique\u2013Universit\u00e9 Grenoble Alpes, 38044 Grenoble Cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvain","family":"Nicolet","sequence":"additional","affiliation":[{"name":"Metalloproteins Unit, Institut de Biologie Structurale Commissariat \u00e0 l'Energie Atomique et aux Energies Renouvelables\u2013Centre National de la Recherche Scientifique\u2013Universit\u00e9 Grenoble Alpes, 38044 Grenoble Cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"Fontecilla-Camps","sequence":"additional","affiliation":[{"name":"Metalloproteins Unit, Institut de Biologie Structurale Commissariat \u00e0 l'Energie Atomique et aux Energies Renouvelables\u2013Centre National de la Recherche Scientifique\u2013Universit\u00e9 Grenoble Alpes, 38044 Grenoble Cedex 9, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,12,22]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr050195z"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr050196r"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5395.1853"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(99)80005-7"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(99)01536-4"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0020963"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12239"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2008.04.063"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001937107"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.09.008"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja1012273"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200907047"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi401143s"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi501205e"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R111.310797"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr9002616"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr4004709"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201402661"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1417252112"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801161200"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.281956"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.07.092"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1246572"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1241859"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/ic102304m"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/ejic.201001101"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1508440112"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045850"},{"key":"e_1_3_3_29_2","first-page":"53","volume-title":"The 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